| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,135,166 | A→G | 68.8% | intergenic (‑139/+153) | pitB ← / ← gss | phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,135,166 | 0 | A | G | 68.8% | 89.1 / 52.5 | 64 | intergenic (‑139/+153) | pitB/gss | phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (15/5); new base G (21/23); total (36/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.79e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATA > W3110S.gb/3135098‑3135230 | gAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaa < 1:1383872/69‑1 (MQ=255)gAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaa > 1:3465238/1‑69 (MQ=255)gAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaa > 1:42728/1‑69 (MQ=255)gAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCaaa < 1:489854/69‑1 (MQ=255) aaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGAc > 1:3119807/1‑69 (MQ=255) tCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGAcc > 1:3325539/1‑66 (MQ=255) tCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAcc > 1:1662590/1‑66 (MQ=255) tCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAcc > 1:1757957/1‑66 (MQ=255) aTTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTa > 1:914762/1‑68 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGa < 1:3456865/68‑1 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGa < 1:378573/68‑1 (MQ=255) ttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat < 1:1984177/68‑1 (MQ=255) cGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATc < 1:2691042/68‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATc < 1:3115874/67‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:2082819/69‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:2157246/69‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:1318150/69‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaa < 1:81813/69‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCa < 1:176161/68‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCa < 1:2391731/68‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCaa < 1:2751882/69‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCa < 1:550930/68‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCa < 1:417768/68‑1 (MQ=255) aTAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaaa < 1:1945226/69‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaaaa < 1:569598/69‑1 (MQ=255) aaaaaGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCaaaa > 1:1929705/1‑68 (MQ=255) aaaaaGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCaaaa > 1:677367/1‑68 (MQ=255) tttgtTATTTTTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGt > 1:3489959/3‑69 (MQ=255) ttATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc < 1:3189559/69‑1 (MQ=255) aTTTGTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAgg > 1:17262/1‑62 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCaaa < 1:710389/36‑1 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAgg > 1:1018239/1‑62 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCa > 1:863888/1‑69 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAgg > 1:2466477/1‑62 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAgg > 1:2454787/1‑62 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAgg > 1:1988065/1‑62 (MQ=255) tttCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTc > 1:2461815/1‑66 (MQ=255) tttCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTc > 1:2623572/1‑66 (MQ=255) tttCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTc > 1:3158795/1‑66 (MQ=255) ttCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc < 1:3660865/65‑1 (MQ=255) cAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAgg > 1:2569256/1‑49 (MQ=255) aGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc > 1:1721078/1‑53 (MQ=255) aGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc > 1:3708262/1‑53 (MQ=255) aGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc > 1:2220787/1‑53 (MQ=255) aGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTc > 1:188384/1‑53 (MQ=255) aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCa > 1:826848/1‑39 (MQ=255) aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCa > 1:97531/1‑39 (MQ=255) aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCa > 1:2132067/1‑39 (MQ=255) aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTcaca > 1:541399/1‑69 (MQ=255) aaGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGt < 1:1320343/42‑1 (MQ=255) aaGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGt > 1:1902896/1‑42 (MQ=255) aaGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:1177423/56‑1 (MQ=255) tcattcaAGACCTTTAGAAATAATCAAAACACAgg < 1:1583480/35‑1 (MQ=255) tcattcaAGACCTTTAGAAATAATCAAAACACAgg < 1:1602181/35‑1 (MQ=255) tcattcaAGACCTTTAGAAATAATCAAAACACAgg < 1:725472/35‑1 (MQ=255) tcattcaAGACCTTTAGAAATAATCAAAACACAgg < 1:626386/35‑1 (MQ=255) tcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg > 1:2007942/1‑69 (MQ=255) tcattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg > 1:3242729/1‑69 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTAt < 1:2787402/37‑1 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTAt < 1:1331321/37‑1 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTAt < 1:2321836/37‑1 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTcc > 1:1991506/1‑40 (MQ=255) cattcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATatgaaatg > 1:1772349/1‑68 (MQ=255) tcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaata > 1:1982090/1‑69 (MQ=255) tcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaata > 1:2600584/1‑69 (MQ=255) tcaAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaata > 1:3067467/1‑69 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt < 1:2864152/39‑1 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTcaca > 1:2229433/1‑55 (MQ=255) caAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTcaca > 1:2233667/1‑55 (MQ=255) | GAAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATA > W3110S.gb/3135098‑3135230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |