| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,138,506 | C→T | 5.8% | G39S (GGT→AGT) | hybG ← | hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,138,506 | 0 | C | T | 5.8% | 219.0 / 3.0 | 69 | G39S (GGT→AGT) | hybG | hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base C (42/23); new base T (2/2); total (44/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
ATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTG > W3110S.gb/3138445‑3138572 | atgCTCATGGCAAATCCGACGTTTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACaa < 1:882099/69‑1 (MQ=255) gCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACaaa < 1:1700157/68‑1 (MQ=255) gCAAATCCGACGTGTACCAGCACCCACTGGCCCATTAGATCGGCAGGGTTACCTTCACAAATCAGGGc > 1:1107192/1‑68 (MQ=255) gCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACTTTCACAGATCAGGGc > 1:1076026/1‑68 (MQ=255) gCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACTTTCACAAATCAGGGc > 1:913097/1‑68 (MQ=255) gCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGc > 1:2404356/1‑68 (MQ=255) gCAAATCCGACGTGTACCAGCACCAACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGc > 1:732053/1‑68 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt > 1:1995101/1‑69 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt > 1:1109193/1‑69 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt > 1:1884281/1‑69 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt > 1:1341354/1‑69 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt > 1:452152/1‑69 (MQ=255) tCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATAtt > 1:308085/1‑69 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCg > 1:1697330/1‑55 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa < 1:3250484/56‑1 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa < 1:2124845/56‑1 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa < 1:510795/56‑1 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa < 1:434893/56‑1 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATcgcg < 1:377890/69‑1 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATcgcg > 1:2136071/1‑69 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATcgcg > 1:142983/1‑69 (MQ=255) tACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCCATATTCACATcgcg < 1:3575915/69‑1 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCgcgc > 1:3557816/1‑68 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCgcgc > 1:3384901/1‑68 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCgcgc > 1:1988623/1‑68 (MQ=255) ccAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCgcgc > 1:1178193/1‑68 (MQ=255) aGCACCCACTGGCCCAGTAGATCGGCAGGGTTACTTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1505167/68‑1 (MQ=255) aGCACCCACTGGCCCAGTAGATCGGCAGGGTTACTTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1100472/68‑1 (MQ=255) aGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1099225/68‑1 (MQ=255) aGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:364767/68‑1 (MQ=255) aGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTg < 1:3606188/69‑1 (MQ=255) ccACTGGCCCAGTAGATCGGCAGGGTTACCTTCACaaa > 1:221470/1‑38 (MQ=255) ccACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAc > 1:1175177/1‑68 (MQ=255) ccACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATAc > 1:2946365/1‑68 (MQ=255) cTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACcaca < 1:187075/69‑1 (MQ=255) ggCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATa > 1:3208561/1‑69 (MQ=255) ggCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATa > 1:2367700/1‑69 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGa > 1:64220/1‑36 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:942589/52‑1 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:1570243/52‑1 (MQ=255) gTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt < 1:2371652/52‑1 (MQ=255) gATCGTCAGGGTTACCTTCACAAATCAGGGCGATAtt > 1:1626140/1‑37 (MQ=255) cGGCAGGGTTACCTTCACAAATCAGGGCGATATTca < 1:797283/36‑1 (MQ=255) cGGCAGGGTTACCTTCACAAATCAGGGCGATATTca < 1:1072600/36‑1 (MQ=255) cGGCAGGGTTACCTTCACAAATCAGGGCGATATTca < 1:3265819/36‑1 (MQ=255) cGGCAGGGTTACCTTCACAAATCAGGGCGATATTca < 1:2043403/36‑1 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATcg > 1:809703/1‑37 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATc > 1:2037480/1‑36 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCtt > 1:1659142/1‑42 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACAt > 1:1882673/1‑52 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACAt < 1:2487943/52‑1 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACAt < 1:1243595/52‑1 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACAt < 1:272636/52‑1 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACAt > 1:79714/1‑52 (MQ=255) aGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACAt < 1:3479399/52‑1 (MQ=255) gTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTg > 1:3145579/1‑69 (MQ=255) gTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTg > 1:2747722/1‑69 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTg > 1:2900536/1‑68 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTg > 1:1525846/1‑68 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTg > 1:558931/1‑68 (MQ=255) ttACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTg > 1:1315373/1‑68 (MQ=255) tACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTgg > 1:628772/1‑68 (MQ=255) tACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTgg > 1:125850/1‑68 (MQ=255) tACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTgg > 1:2625471/1‑68 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACca > 1:1816494/1‑43 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACca > 1:2962014/1‑43 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTg > 1:22121/1‑69 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTg > 1:562974/1‑69 (MQ=255) aCCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTg > 1:1624126/1‑69 (MQ=255) ccTTCACAAATCAGTGCGATATTCACATCGCGCTTGa > 1:1782346/1‑37 (MQ=255) ccTTCACAAATCAGGGCGATATTCACATCGCGCTTGa > 1:1522606/1‑37 (MQ=255) | ATGCTCATGGCAAATCCGACGTGTACCAGCACCCACTGGCCCAGTAGATCGGCAGGGTTACCTTCACAAATCAGGGCGATATTCACATCGCGCTTGATACCACATACTTCAACCTGCGCAAGCTGGTG > W3110S.gb/3138445‑3138572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |