| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,164,775 | C→T | 63.2% | A534T (GCA→ACA) | ygiS ← | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,164,775 | 0 | C | T | 63.2% | 45.9 / 60.0 | 57 | A534T (GCA→ACA) | ygiS | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (13/8); new base T (14/22); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
TCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTA > W3110S.gb/3164710‑3164841 | tCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCtt < 1:1015260/69‑1 (MQ=255)tCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCtt < 1:1890891/69‑1 (MQ=255)tCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCtt < 1:1682553/69‑1 (MQ=255) cacaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTg < 1:1025088/69‑1 (MQ=255) acaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGa > 1:1016204/1‑69 (MQ=255) acaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGa < 1:1525563/69‑1 (MQ=255) acaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGa > 1:75943/1‑69 (MQ=255) aaaGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGa > 1:822905/3‑69 (MQ=255) agaTCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATAc < 1:2447668/69‑1 (MQ=255) gaTCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATAc < 1:1843966/68‑1 (MQ=255) aGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTg < 1:1177539/67‑1 (MQ=255) gcgcCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAg < 1:215735/69‑1 (MQ=255) ccgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATaa < 1:299057/69‑1 (MQ=255) ccgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATa < 1:3095095/68‑1 (MQ=255) cgGACGTGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATaa < 1:2476280/68‑1 (MQ=255) cGTGCTCTGACGGCGATAAAGGCATCAATGTGTCtt > 1:1807905/1‑36 (MQ=37) cGTGCTCCGACGGCGATAAAGGCATCAATGTGTCtt > 1:2582802/1‑36 (MQ=255) tGCTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTggg < 1:2805813/69‑1 (MQ=255) cTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTgggg > 1:942670/1‑68 (MQ=255) cTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTgggg > 1:2858721/1‑68 (MQ=255) cTCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTgggg > 1:1280526/1‑68 (MQ=255) tCCGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTgggg > 1:2908682/1‑67 (MQ=255) ccGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAt < 1:3299304/68‑1 (MQ=255) ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt > 1:1830724/1‑69 (MQ=255) ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt > 1:1750155/1‑69 (MQ=255) ccGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAtt > 1:1745798/1‑69 (MQ=255) cGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACa < 1:1568186/54‑1 (MQ=255) cGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACa < 1:3511916/54‑1 (MQ=255) cGACGGCGATAAAGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACa < 1:1733602/54‑1 (MQ=255) tAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTg < 1:3444932/39‑1 (MQ=255) aaaGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACa < 1:1770215/44‑1 (MQ=255) aaaGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaa < 1:2113437/69‑1 (MQ=255) aaaGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaa < 1:199050/69‑1 (MQ=255) aaGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa > 1:3275267/1‑69 (MQ=255) aaGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa > 1:1118764/1‑69 (MQ=255) aaGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa < 1:499123/69‑1 (MQ=255) aaGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa < 1:1995966/69‑1 (MQ=255) aaGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa > 1:980253/1‑69 (MQ=255) aGGCATCAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGaaa < 1:236996/68‑1 (MQ=255) cAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGcc > 1:3396582/1‑68 (MQ=255) cAATGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGcc > 1:3400777/1‑68 (MQ=255) aaTGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCaa > 1:2219935/1‑69 (MQ=255) aaTGTGTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCa > 1:3719812/1‑68 (MQ=255) aaTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCaa > 1:3300251/1‑69 (MQ=255) aaTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCaa > 1:11999/1‑69 (MQ=255) aaTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCaa > 1:3461785/1‑69 (MQ=255) aaTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCaa > 1:2035514/1‑69 (MQ=255) aaTGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCaa > 1:433219/1‑69 (MQ=255) tgtgTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATg < 1:1563431/50‑1 (MQ=255) tgtgTCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATg < 1:1733564/50‑1 (MQ=255) gtgCCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:1130798/38‑1 (MQ=255) gtgCCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:2753114/38‑1 (MQ=255) gtgCCTTGATATACAACTCTTTGCTGTAGACATAATcc < 1:3304839/38‑1 (MQ=255) gtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGa > 1:2521399/1‑44 (MQ=255) gtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAt > 1:3440666/1‑45 (MQ=255) gtgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGAt > 1:3436375/1‑45 (MQ=255) tgCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTa < 1:1432060/69‑1 (MQ=255) | TCACAGGAGATCAACGTGAATAGCGCCGCCGGACGTGCTCCGACGGCGATAAAGGCATCAATGTGCCTTGATATACAACTCTTTGCTGTAGACATAATCCTGGGGATTATGCAGCGGAAAACCGCCAACGTA > W3110S.gb/3164710‑3164841 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |