| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,194,164 | T→C | 80.9% | T416A (ACG→GCG) | rfaE ← | fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,194,164 | 0 | T | C | 80.9% | 103.9 / 18.8 | 47 | T416A (ACG→GCG) | rfaE | fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (2/7); new base C (19/19); total (21/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.77e-01 | |||||||||||
GGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCAT > W3110S.gb/3194100‑3194221 | ggTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTc < 1:2723772/68‑1 (MQ=255) tataGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCctct > 1:3112549/1‑68 (MQ=255) gccgccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGa < 1:3633953/69‑1 (MQ=255) ccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGacacc < 1:888795/69‑2 (MQ=255) ccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACt < 1:837648/69‑1 (MQ=255) tttCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTa > 1:1653658/1‑68 (MQ=255) tttCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTACTCTTCAAACGACACTa > 1:1000906/1‑68 (MQ=255) tCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCct < 1:4049/51‑1 (MQ=255) tCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCct < 1:913581/51‑1 (MQ=255) tCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:355219/1‑56 (MQ=255) tCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:3274336/1‑56 (MQ=255) tCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:2628232/1‑56 (MQ=255) cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc < 1:2591391/69‑1 (MQ=255) cagcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTc < 1:2801354/69‑1 (MQ=255) gcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:561319/1‑50 (MQ=255) gcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:1580486/1‑50 (MQ=255) gcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:1805260/1‑50 (MQ=255) gcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa < 1:2866231/50‑1 (MQ=255) gcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCCGCGCGTCCTCTTCa < 1:3038363/50‑1 (MQ=39) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCg > 1:3715880/1‑69 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCg > 1:2342726/1‑69 (MQ=255) gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa < 1:1241668/47‑1 (MQ=255) cTGGCAAGATCCCGGGGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt > 1:2247476/1‑68 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:96025/1‑44 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:1335600/1‑44 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCa > 1:1646079/1‑44 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCg < 1:1644809/66‑1 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCg < 1:1042001/66‑1 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt > 1:1049610/1‑68 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt > 1:1299236/1‑68 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt > 1:230799/1‑68 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt > 1:2649633/1‑68 (MQ=255) cTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCt > 1:1481825/1‑68 (MQ=255) aGATCCCGGCTATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc < 1:3452927/64‑1 (MQ=255) aGATCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc < 1:2649919/64‑1 (MQ=255) tCCCGGCGATCAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTc < 1:1206924/61‑1 (MQ=255) cccGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTcc < 1:3562410/61‑1 (MQ=255) cccGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTcc < 1:2925809/61‑1 (MQ=255) ggCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAg < 1:634155/69‑1 (MQ=255) cAAGGGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAAt < 1:1249539/69‑1 (MQ=255) cAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAAt > 1:404445/1‑69 (MQ=255) cAAGCGCTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAAt < 1:1087153/69‑1 (MQ=255) aaGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAAt < 1:3085373/68‑1 (MQ=255) gcgcTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCAt < 1:2760283/69‑1 (MQ=255) gcgcTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCAt < 1:3496753/69‑1 (MQ=255) gcgcTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCAt < 1:237548/69‑1 (MQ=255) gcgcTGCGGCGCGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCAt < 1:2935865/69‑1 (MQ=255) | GGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCAT > W3110S.gb/3194100‑3194221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |