| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,198,610 | A→G | 58.3% | F338L (TTC→CTC) | ygiF ← | predicted adenylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,198,610 | 0 | A | G | 58.3% | 18.6 / 40.8 | 36 | F338L (TTC→CTC) | ygiF | predicted adenylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (6/9); new base G (6/15); total (12/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAAC > W3110S.gb/3198545‑3198674 | cTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGa < 1:91570/69‑1 (MQ=255) gAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCt < 1:111858/69‑1 (MQ=255) gAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCt < 1:24161/69‑1 (MQ=255) gAAAACGCTTTTCAGTTCAGCGGCATGACGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCt < 1:1180225/69‑1 (MQ=255) gAAAACGCTTTTCAGTTCAGCGGCATGACGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCt < 1:3670665/69‑1 (MQ=255) gAAAACGCTTTTCAGTTCAGCGGCATGACGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCt < 1:2226602/69‑1 (MQ=255) aaaCGCTTTTCAGTTCAGCGGCATAGCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGa > 1:1641904/1‑69 (MQ=255) aaaCGCTTTTCAGTTCAGAGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGa > 1:2569044/1‑69 (MQ=255) aaCGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGAt < 1:1976260/69‑1 (MQ=255) cGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGAttt < 1:1856962/69‑1 (MQ=255) ttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTGGCTGATTTTGCCCTgcgc < 1:2902672/69‑1 (MQ=255) ttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTgcgc < 1:1512472/69‑1 (MQ=255) ttcagCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTgcgc < 1:1126021/69‑1 (MQ=255) ggCGGGAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTa < 1:1004344/69‑1 (MQ=255) gCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTa > 1:1969140/1‑68 (MQ=255) gggAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaaa < 1:3561228/68‑1 (MQ=255) gAAAGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAAt < 1:2860428/69‑1 (MQ=255) aaaGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTg > 1:668525/1‑56 (MQ=255) aaaGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATg > 1:121888/1‑69 (MQ=255) aaaGATGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATg > 1:1363262/1‑69 (MQ=255) aaaGAAGGATATCGGCAAAGCGGTTGAGGGAGTCGCTGAatt > 1:2045447/1‑42 (MQ=25) aGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTg < 1:1224372/54‑1 (MQ=255) tGGATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGc < 1:2708831/69‑1 (MQ=255) tGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGc > 1:3065036/1‑69 (MQ=255) tGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGc > 1:495294/1‑69 (MQ=255) gATATCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCa < 1:2000529/69‑1 (MQ=255) tatCGGCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtg < 1:3327285/69‑1 (MQ=255) atCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCAtgc > 1:764497/1‑69 (MQ=255) ggCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaaa < 1:3036775/52‑1 (MQ=255) ggCAAAGCGTTTGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaaa < 1:864978/52‑1 (MQ=255) aGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATATAAAAATGGCTGcc > 1:33497/1‑57 (MQ=255) aGCGTTTGAAGGAGTCGCTGATTTTGACCTGCGCTTTGGCATc > 1:1745737/1‑43 (MQ=255) ttGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTaccaac < 1:1218253/69‑1 (MQ=255) ttGAGGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTaccaac < 1:2133514/69‑1 (MQ=255) tGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaaa < 1:2655147/41‑1 (MQ=255) tGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTaaa < 1:99366/41‑1 (MQ=255) | CTGGCAGAAAACGCTTTTCAGTTCAGCGGCATGGCGGGAAAGATGGATATCGGCAAAGCGTTTGAAGGAGTCGCTGATTTTGCCCTGCGCTTTGGCATCTAAAAATGGCTGCCATGCTTTGCTTACCAAC > W3110S.gb/3198545‑3198674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |