| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,205,103 | A→G | 79.5% | intergenic (‑191/‑16) | ygiP ← / → ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,205,103 | 0 | A | G | 79.5% | 81.4 / 15.6 | 39 | intergenic (‑191/‑16) | ygiP/ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (17/14); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG > W3110S.gb/3205042‑3205166 | aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTg < 1:3529724/69‑1 (MQ=255) aaaaTATAAAACCACATTTTTAGTGGTAGTTTGAGGCGGTGAATTTTTCCAGACAAATACGAAAACTgg < 1:1771641/69‑1 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCatgatg < 1:1295481/69‑1 (MQ=255) acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCatgatg < 1:159802/69‑1 (MQ=255) aTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg < 1:3092473/69‑1 (MQ=255) tttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAg > 1:3461001/1‑66 (MQ=255) tttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGc > 1:742957/1‑67 (MQ=255) tttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGc > 1:1052967/1‑67 (MQ=255) tGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:1994900/68‑1 (MQ=255) gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:3039657/66‑1 (MQ=255) gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:1188987/66‑1 (MQ=255) aGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata > 1:346584/1‑68 (MQ=255) aGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata > 1:2354256/1‑68 (MQ=255) aGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaata > 1:2145951/1‑68 (MQ=255) tgtgGCGGTGAATTTTTCCAGAGAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt < 1:52850/60‑1 (MQ=255) tgtgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGa > 1:2996942/1‑39 (MQ=255) tgtgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGATAGtaat < 1:475751/63‑1 (MQ=255) tgtgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 1:1791756/63‑1 (MQ=255) tgtgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 1:987497/63‑1 (MQ=255) gtgGCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGt > 1:161445/1‑59 (MQ=255) gCGGTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg < 1:2429686/69‑1 (MQ=255) ggTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 1:561698/68‑1 (MQ=255) gTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa > 1:2443584/1‑68 (MQ=255) gTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa > 1:57954/1‑68 (MQ=255) gTGAATTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAAAGGCa > 1:2139406/1‑68 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAg > 1:2376535/1‑49 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAg > 1:399498/1‑49 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAg > 1:2104416/1‑49 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAg > 1:185568/1‑49 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAg > 1:1510366/1‑49 (MQ=255) aaTTTTTCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGtaat < 1:3365013/53‑1 (MQ=255) ttttCCAGAGAAATAGGAAAACTGGAGTTGCCatgatg < 1:656955/38‑1 (MQ=25) ttttCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa > 1:1676053/1‑69 (MQ=255) ttCCAGACAAATACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg > 1:1125127/1‑69 (MQ=255) aCAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa < 1:2830137/69‑1 (MQ=255) aaaTACGAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGa > 1:679866/1‑65 (MQ=255) aaaTACGAAAACTGGAGTTGCCATGATGAGCGAAAGT‑ATAAGCAACAGGCAGTGAATAAGTTGa > 1:360594/1‑64 (MQ=255) aaTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag < 1:2048147/69‑1 (MQ=255) aTACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACagag < 1:2610919/68‑1 (MQ=255) | AAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG > W3110S.gb/3205042‑3205166 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |