| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,219,258 | T→C | 18.9% | F379S (TTC→TCC) | ygjG → | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,219,258 | 0 | T | C | 18.9% | 115.8 / 22.5 | 53 | F379S (TTC→TCC) | ygjG | putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (24/19); new base C (2/8); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
AATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTT > W3110S.gb/3219191‑3219318 | aaTGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGttt > 1:2849511/1‑68 (MQ=255)aaTGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTc > 1:92978/1‑69 (MQ=255)aaTGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTc > 1:889797/1‑69 (MQ=255) tgtgTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTc > 1:1971379/1‑67 (MQ=255) tgtgTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTc > 1:3514414/1‑67 (MQ=255) tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGt > 1:1871086/1‑68 (MQ=255) tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTc < 1:894198/69‑1 (MQ=255) tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTc > 1:1423665/1‑69 (MQ=255) tgtTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTc < 1:2857468/69‑1 (MQ=255) ttGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCa < 1:211573/68‑1 (MQ=255) aaCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGaa < 1:306570/69‑1 (MQ=255) aaCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCggg < 1:2574450/67‑1 (MQ=255) aCTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAat > 1:1273251/1‑69 (MQ=255) cTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGa > 1:2447013/1‑66 (MQ=255) cTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAata < 1:2922288/69‑1 (MQ=255) ttACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat < 1:438150/69‑1 (MQ=255) ttACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAatat < 1:2369992/69‑1 (MQ=255) ttACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAata < 1:1885707/68‑1 (MQ=255) tACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATc > 1:2364897/1‑69 (MQ=255) tACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATc > 1:3097024/1‑69 (MQ=255) tACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATc > 1:894536/1‑69 (MQ=255) tACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATc > 1:1165009/1‑69 (MQ=255) aCCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATc > 1:188649/1‑68 (MQ=255) ggCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGa > 1:986613/1‑69 (MQ=255) ggCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGa > 1:2459702/1‑69 (MQ=255) aGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATccc > 1:3243285/1‑62 (MQ=255) gCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTgg < 1:2863422/67‑1 (MQ=255) tGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTAc > 1:1394615/1‑68 (MQ=255) tGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATATGGTAc > 1:1401874/1‑68 (MQ=255) aaaaaGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAg > 1:1400171/1‑69 (MQ=255) aGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGcgcg < 1:689120/69‑1 (MQ=255) aGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGcgcg > 1:2349823/1‑69 (MQ=255) aGGCGATATGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGcgcg > 1:167306/1‑69 (MQ=255) atatGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTa < 1:1786750/69‑1 (MQ=255) atatGTTGCTGGACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTa < 1:1254240/69‑1 (MQ=255) ttGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAggg > 1:779735/1‑69 (MQ=255) ttGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAggg > 1:437106/1‑69 (MQ=255) ttGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAggg > 1:1459855/1‑69 (MQ=255) ttGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAggg > 1:3103282/1‑69 (MQ=255) ttGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAggg > 1:3443072/1‑69 (MQ=255) gCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGa < 1:3423210/68‑1 (MQ=255) ggACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:56458/69‑1 (MQ=255) ggACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1458015/69‑1 (MQ=255) ggACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1871242/69‑1 (MQ=255) ggACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:152877/69‑1 (MQ=255) ggACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1688146/69‑1 (MQ=255) ggACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:2828735/69‑1 (MQ=255) ggACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:2782833/69‑1 (MQ=255) ggACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1014457/69‑1 (MQ=255) ggACGGTTCCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1018632/69‑1 (MQ=255) cGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:3082800/66‑1 (MQ=255) cGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:2492452/66‑1 (MQ=255) cGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1391366/66‑1 (MQ=255) ggTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGtt < 1:1489365/65‑1 (MQ=255) ttCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTa < 1:2924698/52‑1 (MQ=255) | AATGTGTTGCTGGAGCAGAACTTACCGGCTCAGGCTGAGCAAAAAGGCGATATGTTGCTGGACGGTTTCCGTCAACTGGCGCGGGAATATCCCGATCTGGTACAGGAAGCGCGTGGTAAAGGGATGTT > W3110S.gb/3219191‑3219318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |