| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 15,286 | C→T | 61.5% | D373D (GAC→GAT) | dnaJ → | chaperone Hsp40, co‑chaperone with DnaK |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 15,286 | 0 | C | T | 61.5% | 14.4 / 26.6 | 26 | D373D (GAC→GAT) | dnaJ | chaperone Hsp40, co‑chaperone with DnaK |
| Reads supporting (aligned to +/- strand): ref base C (7/3); new base T (6/10); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
CCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTG > W3110S.gb/15222‑15345 | ccGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTgt < 1:500701/69‑2 (MQ=255)ccGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGa < 1:863896/69‑1 (MQ=255)ccGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGa < 1:243956/69‑1 (MQ=255)ccGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGa > 1:2729180/1‑69 (MQ=255) aGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCt < 1:255543/69‑1 (MQ=255) gCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTa < 1:1128336/69‑1 (MQ=255) gCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTa < 1:3091172/69‑1 (MQ=255) acaacaGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTAAc < 1:2117863/69‑1 (MQ=255) caGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTa > 1:2043749/1‑63 (MQ=255) caGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTa > 1:2954278/1‑63 (MQ=255) gcgcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTAACCTCCCCaaaa < 1:1078504/69‑1 (MQ=255) gcgcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTAACCTCCCCaaaa < 1:1074059/69‑1 (MQ=255) cgcTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGATCTGACCCGCTAACCTCCCCaaaa < 1:1295203/68‑1 (MQ=255) aaaGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGc > 1:3678471/1‑69 (MQ=255) cttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCa > 1:271551/1‑69 (MQ=255) cttTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCa > 1:2759324/1‑69 (MQ=255) gtgtGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTg > 1:1979791/1‑67 (MQ=255) aagaagTTTTTTGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTaaa < 1:726632/68‑1 (MQ=255) agaagTTTTTTGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTaaa < 1:701753/67‑1 (MQ=255) agTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAg < 1:2035974/69‑1 (MQ=255) gTTTTTTGACGATCTGACCCGCTAACCTCCCCGAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg > 1:2068755/1‑69 (MQ=255) gTTTTTTGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAgg > 1:359754/1‑69 (MQ=255) ttttGACGATCTGACCCGCTAACCTCCCCAAAAGCCTg > 1:1210033/1‑38 (MQ=255) ttttGACGATCTGACCCGCTAACCTCCCCAAAAGCCTg > 1:705538/1‑38 (MQ=255) ttttGACGATCTGACCCGCTAACCTCCCCAAAAGCCTg > 1:2109636/1‑38 (MQ=255) ttttGACGATCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTg > 1:1998841/1‑69 (MQ=255) | CCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTG > W3110S.gb/15222‑15345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |