Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 230,040 C→A 100% R281L (CGC→CTC)  yafC ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb230,0400CA80.1% 41.0 / 14.3 30R281L (CGC→CTC) yafCpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (5/1);  new base A (12/12);  total (17/13)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCT  >  W3110S.gb/229981‑230108
                                                           |                                                                    
cAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTa                                                             >  1:2832872/1‑69 (MQ=255)
   ctcctcCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCg                                                          <  1:3002486/69‑1 (MQ=255)
         cGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt                                                                  <  1:625976/55‑1 (MQ=255)
         cGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGt                                                    >  1:1934190/1‑69 (MQ=255)
              gCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg                                               <  1:1482505/69‑1 (MQ=255)
               cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt                                              >  1:2320537/1‑69 (MQ=255)
                tGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCg                                                          <  1:677523/56‑1 (MQ=255)
                tGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCg                                                          >  1:3537270/1‑56 (MQ=255)
                    tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACt                                                               >  1:1948213/1‑47 (MQ=255)
                    tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACt                                                               >  1:2944272/1‑47 (MQ=255)
                    tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACt                                                               >  1:2299556/1‑47 (MQ=255)
                    tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa                                          <  1:150291/68‑1 (MQ=255)
                    tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa                                          <  1:2628060/68‑1 (MQ=255)
                     ttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAg                                         <  1:151388/68‑1 (MQ=255)
                          tGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACgg                                     >  1:2937520/1‑67 (MQ=255)
                          tGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACgg                                     >  1:1683831/1‑67 (MQ=255)
                           gCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt                                                                  >  1:1690283/1‑37 (MQ=255)
                           gCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt                                                                  >  1:29846/1‑37 (MQ=255)
                           gCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt                                                                  >  1:3362649/1‑37 (MQ=255)
                              cGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt                                              <  1:2982800/54‑1 (MQ=255)
                              cGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg                                <  1:3061631/68‑1 (MQ=255)
                              cGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg                                <  1:2012810/68‑1 (MQ=255)
                                  aaGGAAATCGATAAAAGACCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg                           <  1:2330523/69‑1 (MQ=255)
                                           gATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc                     >  1:3239141/1‑66 (MQ=255)
                                           gATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc                     >  1:1065479/1‑66 (MQ=255)
                                             tAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACt                 <  1:217637/68‑1 (MQ=255)
                                             tAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACt                 <  1:201943/68‑1 (MQ=255)
                                                 aggccGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCaa            >  1:1594121/4‑69 (MQ=255)
                                                 aGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCaa            >  1:2382263/1‑69 (MQ=255)
                                                 aGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCaa            >  1:462779/1‑69 (MQ=255)
                                                           cgcgTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCt  >  1:1574012/1‑69 (MQ=255)
                                                           |                                                                    
CAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCT  >  W3110S.gb/229981‑230108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: