| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 230,040 | C→A | 100% | R281L (CGC→CTC) | yafC ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 230,040 | 0 | C | A | 80.1% | 41.0 / 14.3 | 30 | R281L (CGC→CTC) | yafC | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (5/1); new base A (12/12); total (17/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCT > W3110S.gb/229981‑230108 | cAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTa > 1:2832872/1‑69 (MQ=255) ctcctcCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCg < 1:3002486/69‑1 (MQ=255) cGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt < 1:625976/55‑1 (MQ=255) cGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGt > 1:1934190/1‑69 (MQ=255) gCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg < 1:1482505/69‑1 (MQ=255) cTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt > 1:2320537/1‑69 (MQ=255) tGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCg < 1:677523/56‑1 (MQ=255) tGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCg > 1:3537270/1‑56 (MQ=255) tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACt > 1:1948213/1‑47 (MQ=255) tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACt > 1:2944272/1‑47 (MQ=255) tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACt > 1:2299556/1‑47 (MQ=255) tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:150291/68‑1 (MQ=255) tttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATa < 1:2628060/68‑1 (MQ=255) ttACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAg < 1:151388/68‑1 (MQ=255) tGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACgg > 1:2937520/1‑67 (MQ=255) tGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACgg > 1:1683831/1‑67 (MQ=255) gCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt > 1:1690283/1‑37 (MQ=255) gCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt > 1:29846/1‑37 (MQ=255) gCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGt > 1:3362649/1‑37 (MQ=255) cGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:2982800/54‑1 (MQ=255) cGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:3061631/68‑1 (MQ=255) cGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:2012810/68‑1 (MQ=255) aaGGAAATCGATAAAAGACCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATgg < 1:2330523/69‑1 (MQ=255) gATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc > 1:3239141/1‑66 (MQ=255) gATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTc > 1:1065479/1‑66 (MQ=255) tAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACt < 1:217637/68‑1 (MQ=255) tAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACt < 1:201943/68‑1 (MQ=255) aggccGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCaa > 1:1594121/4‑69 (MQ=255) aGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCaa > 1:2382263/1‑69 (MQ=255) aGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCaa > 1:462779/1‑69 (MQ=255) cgcgTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCt > 1:1574012/1‑69 (MQ=255) | CAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCT > W3110S.gb/229981‑230108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |