| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,232,470 | A→G | 11.8% | L111L (TTG→CTG) | ygjM ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,232,470 | 0 | A | G | 11.8% | 133.7 / 10.2 | 51 | L111L (TTG→CTG) | ygjM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (19/26); new base G (2/4); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTG > W3110S.gb/3232405‑3232533 | agaAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa > 1:2187867/1‑66 (MQ=255)agaAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa > 1:3057224/1‑66 (MQ=255)agaAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc > 1:2241266/1‑69 (MQ=255)agaAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc > 1:3248534/1‑69 (MQ=255)agaAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc > 1:177869/1‑69 (MQ=255)agaAATGCCGAATCGCGTTGCCAATTTTTAAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAAc > 1:2363230/1‑69 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGt > 1:2902515/1‑69 (MQ=255) gCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGa > 1:1850850/1‑69 (MQ=255) gCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGa > 1:3442809/1‑69 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt < 1:3119701/66‑1 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt < 1:1994978/66‑1 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt < 1:394589/66‑1 (MQ=255) aTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa < 1:3125608/69‑1 (MQ=255) aTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa < 1:1487871/69‑1 (MQ=255) aTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCa < 1:899556/69‑1 (MQ=255) gTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGaca > 1:1134435/1‑61 (MQ=255) gTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGaca > 1:2329651/1‑61 (MQ=255) ttGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:632607/69‑1 (MQ=255) ttGCCAA‑TTTTTATCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAttt < 1:3232920/69‑1 (MQ=255) tGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAttt < 1:2881971/69‑1 (MQ=255) tGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:2504050/68‑1 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAttt < 1:2757215/68‑1 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTa > 1:2478315/1‑69 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTa > 1:3305910/1‑69 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTa > 1:1807233/1‑69 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTa > 1:1829205/1‑69 (MQ=255) aTTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGacac > 1:1913341/1‑55 (MQ=255) ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaa < 1:2976099/42‑1 (MQ=255) tttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCa > 1:2721091/1‑69 (MQ=255) tttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCa < 1:1816134/69‑1 (MQ=255) tttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCa < 1:1562651/69‑1 (MQ=255) ttCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTcc > 1:812059/1‑64 (MQ=255) cGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATATAtt < 1:63542/44‑1 (MQ=255) gTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:1491385/43‑1 (MQ=255) gTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:3640598/43‑1 (MQ=255) gTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:3626453/43‑1 (MQ=255) gTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:639339/43‑1 (MQ=255) aaTTTCCTCTTCCCTCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAgg > 1:983660/1‑69 (MQ=255) tCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCa > 1:355347/1‑44 (MQ=255) tCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCa > 1:2661297/1‑44 (MQ=255) tCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAg > 1:3043348/1‑64 (MQ=255) tCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTa < 1:3006109/69‑1 (MQ=255) ccTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:497798/35‑1 (MQ=255) ccTCTTCCCGCTCAAAACGCGTGACACCATAGAtt < 1:278175/35‑1 (MQ=255) ctTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAAcc < 1:1064111/69‑1 (MQ=255) ctTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAAc < 1:3203378/68‑1 (MQ=255) cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa < 1:2148771/67‑1 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:3116042/1‑69 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa < 1:1559658/67‑1 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa < 1:1884865/67‑1 (MQ=255) cccGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa < 1:2497426/67‑1 (MQ=255) gCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTg < 1:420917/69‑1 (MQ=255) gCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTg < 1:945513/69‑1 (MQ=255) cAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCat < 1:3614459/62‑1 (MQ=255) | AGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTG > W3110S.gb/3232405‑3232533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |