| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,272,176 | T→C | 31.5% | D146G (GAC→GGC) | garR ← | tartronate semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,272,176 | 0 | T | C | 31.5% | 92.2 / 64.9 | 73 | D146G (GAC→GGC) | garR | tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base T (26/24); new base C (15/8); total (41/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGC > W3110S.gb/3272109‑3272242 | aCCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGt > 1:238844/1‑68 (MQ=255)aCCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGt > 1:2203027/1‑68 (MQ=255)aCCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGt > 1:1534804/1‑68 (MQ=255)aCCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGt > 1:506881/1‑68 (MQ=255) gCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa > 1:1445232/1‑68 (MQ=255) cACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa > 1:844486/1‑67 (MQ=255) gATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGc < 1:3378562/69‑1 (MQ=255) gATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc < 1:1252945/69‑1 (MQ=255) gATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc > 1:2306037/1‑69 (MQ=255) gATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc > 1:3707727/1‑69 (MQ=255) gATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc > 1:3431012/1‑69 (MQ=255) gATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGc > 1:174694/1‑69 (MQ=255) aTTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGcc > 1:2284718/1‑69 (MQ=255) ttCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt < 1:522267/69‑1 (MQ=255) tCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTg > 1:3332681/1‑69 (MQ=255) tCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTg > 1:1706957/1‑69 (MQ=255) tCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTg > 1:3019713/1‑69 (MQ=255) tATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt < 1:3621829/61‑1 (MQ=255) tATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt < 1:1092638/61‑1 (MQ=255) aTGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt > 1:999765/1‑60 (MQ=255) aTGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt > 1:2205013/1‑60 (MQ=255) aTGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt > 1:3359752/1‑60 (MQ=255) aTGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt < 1:3456451/60‑1 (MQ=255) tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaaa < 1:3313866/52‑1 (MQ=255) tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa > 1:2830388/1‑52 (MQ=255) tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa > 1:2561734/1‑52 (MQ=255) tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa < 1:3394595/52‑1 (MQ=255) tGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGaaaa < 1:1317868/52‑1 (MQ=255) caccacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTc < 1:2987598/60‑1 (MQ=255) caccacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCcca < 1:327723/68‑1 (MQ=255) cacGGAACCCGCCGTCGCTTTCATCAAATCATAGTATTTGTCGaa > 1:3633473/1‑45 (MQ=255) cacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa > 1:1506430/1‑45 (MQ=255) cacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa > 1:403331/1‑45 (MQ=255) cacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa > 1:3293416/1‑45 (MQ=255) cacGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCAt > 1:1005180/1‑69 (MQ=255) acGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGccc > 1:2352592/1‑63 (MQ=255) acGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATc > 1:3198427/1‑69 (MQ=255) ggAACCCGCCATCGCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCtt < 1:3100892/51‑1 (MQ=255) aaCCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaaa < 1:1394571/42‑1 (MQ=255) aCCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaaa > 1:1652468/1‑41 (MQ=255) ccGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCtt < 1:3472648/46‑1 (MQ=255) cATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGt < 1:736863/69‑1 (MQ=255) cATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGt < 1:3147329/69‑1 (MQ=255) cGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATc > 1:1605114/1‑55 (MQ=255) cGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATc > 1:2343924/1‑55 (MQ=255) gCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACt < 1:1144192/57‑1 (MQ=255) gCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACt < 1:3313006/57‑1 (MQ=255) gCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACt < 1:738690/57‑1 (MQ=255) gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTcgc < 1:665175/43‑1 (MQ=255) gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCccac < 1:3432317/50‑1 (MQ=255) gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCg > 1:3286403/1‑69 (MQ=255) gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCg > 1:408128/1‑69 (MQ=255) gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCg > 1:3034029/1‑69 (MQ=255) gCTTTCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCg > 1:3453285/1‑69 (MQ=255) tttCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc < 1:2043536/69‑1 (MQ=255) ttCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCg > 1:983261/1‑69 (MQ=255) ttCATCAAATCATAGTATTTGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCg > 1:1693492/1‑69 (MQ=255) atcaAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATg > 1:219941/1‑69 (MQ=255) caAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGc < 1:413406/69‑1 (MQ=255) caAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGc < 1:1083770/69‑1 (MQ=255) tCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTc > 1:2360286/1‑69 (MQ=255) tCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTc > 1:2629732/1‑69 (MQ=255) gTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGAt < 1:3239831/57‑1 (MQ=255) gTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTc > 1:1516127/1‑69 (MQ=255) gTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTc > 1:2935952/1‑69 (MQ=255) tttGCCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc > 1:523680/1‑51 (MQ=255) tttGCCGAAAATAGCCTTGTCGC‑‑CCCACCATCACTGACAGCGTACCGTc > 1:2553048/1‑49 (MQ=255) tttGCCGAAAATAGCCTTGACGCCGCCCACCATCACTGACAGCGTACCGTc > 1:2074619/1‑51 (MQ=38) ttGTCGAAAATAGCCTTGTCGCCGCCCACCATCACt < 1:1193538/36‑1 (MQ=255) ttGTCGAAAATAGCCTTGTCGCCGCCCACCATCACt < 1:1101537/36‑1 (MQ=255) ttGTCGAAAATAGCCTTGTCGCCGCCCACCATCACt < 1:3329672/36‑1 (MQ=255) tGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc < 1:330252/49‑1 (MQ=255) tGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc < 1:2111642/49‑1 (MQ=255) tGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc > 1:2792973/1‑49 (MQ=255) tGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTc < 1:1604005/49‑1 (MQ=255) tGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCAccgc > 1:360377/1‑69 (MQ=255) gTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTc < 1:133328/63‑1 (MQ=255) | ACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGC > W3110S.gb/3272109‑3272242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |