| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,343,341 | T→C | 26.1% | I36T (ATC→ACC) | yhbN → | predicted transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,343,341 | 0 | T | C | 26.1% | 115.3 / 48.2 | 69 | I36T (ATC→ACC) | yhbN | predicted transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (21/30); new base C (10/8); total (31/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC > W3110S.gb/3343282‑3343408 | cTCACTTCTGGCCGCCCGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGa < 1:467265/69‑1 (MQ=255) tCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGaa < 1:2152166/69‑1 (MQ=255) tCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg > 1:2141446/1‑68 (MQ=255) tCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg > 1:1050030/1‑68 (MQ=255) cTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGCCCCACATTGAATCgg > 1:183410/1‑67 (MQ=255) cTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCgg > 1:2234646/1‑67 (MQ=255) tGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGAcc > 1:1143624/1‑69 (MQ=255) tGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGAcc > 1:1855208/1‑69 (MQ=255) cgccAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCa < 1:3258587/69‑1 (MQ=255) aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca > 1:3283469/1‑47 (MQ=255) aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca > 1:2808835/1‑47 (MQ=255) aGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCca > 1:3642300/1‑47 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg < 1:31256/53‑1 (MQ=255) tCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCgg < 1:2450187/53‑1 (MQ=255) ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat > 1:1416615/1‑69 (MQ=255) ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat > 1:867581/1‑69 (MQ=255) ccGGCATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGat > 1:37162/1‑69 (MQ=255) cATTTGCCGTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGatat > 1:1476464/1‑67 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGa > 1:34397/1‑39 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:3338426/1‑43 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:3194954/1‑43 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:2465797/1‑43 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCg > 1:2466474/1‑43 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg > 1:947783/1‑68 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg > 1:1391626/1‑68 (MQ=255) tGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAg > 1:1987831/1‑68 (MQ=255) gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTg > 1:450745/1‑55 (MQ=255) gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCaa < 1:3023920/68‑1 (MQ=255) gTAACCGGAGACACTGATCAGCCGACCCACATTGAATCGGACAAGCAAtct > 1:1677662/1‑51 (MQ=255) cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:2063728/68‑1 (MQ=255) cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:2561274/68‑1 (MQ=255) cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:1162525/68‑1 (MQ=255) cGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTgg < 1:642105/68‑1 (MQ=255) cGGAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGt < 1:1700767/69‑1 (MQ=255) ggAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGtt < 1:3677516/69‑1 (MQ=255) ggAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGtt < 1:644465/69‑1 (MQ=255) ggAGACACTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGtt < 1:1870891/69‑1 (MQ=255) agaCACTGATCAGCCTATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1638503/69‑1 (MQ=255) agaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1631591/69‑1 (MQ=255) gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc < 1:1858571/69‑1 (MQ=255) gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc < 1:1901491/69‑1 (MQ=255) gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1635776/68‑1 (MQ=255) gaCACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAc < 1:1527876/68‑1 (MQ=255) acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc > 1:2536496/1‑68 (MQ=255) acacTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTAcc > 1:3330352/1‑68 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCaa > 1:2254343/1‑36 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCaa > 1:3636722/1‑36 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:723998/45‑1 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:568683/45‑1 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:1362960/45‑1 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:3685169/45‑1 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGat < 1:2081066/45‑1 (MQ=255) acTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCttt < 1:3012187/69‑1 (MQ=255) cTGATCAGCCGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCttt < 1:2043389/68‑1 (MQ=255) gATCAGCCGATCCACATTGAATCGGCCCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:758944/1‑69 (MQ=255) gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGc < 1:297941/51‑1 (MQ=255) gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAcc > 1:3672433/1‑69 (MQ=255) gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTAc > 1:2768357/1‑68 (MQ=255) gATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTT‑ATATGCAAGGCAACGTGGTTACCTTTAc > 1:1325264/1‑67 (MQ=255) cAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGt < 1:3348527/69‑1 (MQ=255) ccGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAAt < 1:3668280/69‑1 (MQ=255) cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:3446294/49‑1 (MQ=255) cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:703188/49‑1 (MQ=255) cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:2700261/49‑1 (MQ=255) cGACCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGt < 1:975818/49‑1 (MQ=255) aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:566460/69‑1 (MQ=255) aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:3307631/69‑1 (MQ=255) aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:281880/69‑1 (MQ=255) aTCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTc < 1:2692158/69‑1 (MQ=255) | CTCACTTCTGGCCGCCAGCATTCCGGCATTTGCCGTAACCGGAGACACTGATCAGCCGATCCACATTGAATCGGACCAGCAATCTCTTGATATGCAAGGCAACGTGGTTACCTTTACCGGTAATGTC > W3110S.gb/3343282‑3343408 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |