| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,347,872 | C→T | 24.5% | R18W (CGG→TGG) | npr → | phosphohistidinoprotein‑hexose phosphotransferase component of N‑regulated PTS system (Npr) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,347,872 | 0 | C | T | 24.5% | 91.6 / 26.5 | 49 | R18W (CGG→TGG) | npr | phosphohistidinoprotein‑hexose phosphotransferase component of N‑regulated PTS system (Npr) |
| Reads supporting (aligned to +/- strand): ref base C (16/20); new base T (6/6); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTACGTAATGACG > W3110S.gb/3347805‑3347938 | tGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg < 1:568186/69‑1 (MQ=255)tGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg < 1:544015/69‑1 (MQ=255)tGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg < 1:3423923/69‑1 (MQ=255)tGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg < 1:3171740/69‑1 (MQ=255)tGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg < 1:290875/69‑1 (MQ=255)tGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg < 1:2715935/69‑1 (MQ=255)tGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCg < 1:2487460/69‑1 (MQ=255) tGACCGACAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGt > 1:8274/1‑69 (MQ=255) aCCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGtt > 1:1901827/1‑68 (MQ=255) aCCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGtt > 1:1911097/1‑68 (MQ=255) cGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTg < 1:2481805/68‑1 (MQ=255) cAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAAtt > 1:2134485/1‑69 (MQ=255) cAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAAtt < 1:2352265/69‑1 (MQ=255) gCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTg < 1:2989691/62‑1 (MQ=255) cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg < 1:235488/51‑1 (MQ=255) cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg < 1:2382012/51‑1 (MQ=255) cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATg < 1:1432182/51‑1 (MQ=255) cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTaa > 1:181014/1‑67 (MQ=255) cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTaa > 1:3550191/1‑67 (MQ=255) cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTaa > 1:3331482/1‑67 (MQ=255) cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTaa > 1:2219703/1‑67 (MQ=255) aaCTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCa > 1:1872761/1‑69 (MQ=255) gTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGt > 1:2914001/1‑69 (MQ=255) gAAATCACAAACAAGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt < 1:2910037/69‑1 (MQ=255) gAAATCACAAACAAGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt < 1:2278851/69‑1 (MQ=255) gAAATCACAAACAAGCTGGGCATGCATGCCCGTCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt < 1:822848/69‑1 (MQ=255) gAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt < 1:1079918/69‑1 (MQ=255) gAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt < 1:1225785/69‑1 (MQ=255) gAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt < 1:857969/69‑1 (MQ=255) gAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt < 1:2042845/69‑1 (MQ=255) cacaAACAAGCTGGGCATGCATGCCTGGCCTGCAAt < 1:2081024/36‑1 (MQ=255) cacaAACAAGCTGGGCATGCATGCCTGGCCTGCAAt < 1:3394205/36‑1 (MQ=255) acaaacaaGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGc > 1:385928/1‑68 (MQ=255) acaaacaaGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCt < 1:3713271/69‑1 (MQ=255) aacaaGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTg < 1:1379773/67‑1 (MQ=255) aaGCTGGGGATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTg > 1:3400252/1‑69 (MQ=255) aaGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTg > 1:2004324/1‑69 (MQ=255) aaGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTg > 1:796442/1‑69 (MQ=255) aGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTg > 1:1363633/1‑63 (MQ=255) aGCTGGGCATGCATGCCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTg > 1:1164161/1‑63 (MQ=255) gCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTg < 1:717277/38‑1 (MQ=255) tGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAAtt > 1:3295633/1‑40 (MQ=255) tGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAAtt > 1:1670849/1‑40 (MQ=255) gcatgcCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt > 1:1312775/1‑46 (MQ=255) gcatgcCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt > 1:3100618/1‑46 (MQ=255) gcatgcCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt > 1:54204/1‑46 (MQ=255) gcatgcCAGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt > 1:1158165/1‑46 (MQ=255) catgcCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt > 1:657374/1‑45 (MQ=255) catgcCTGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTACGTAAt < 1:2842280/69‑1 (MQ=255) cccGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGtttt > 1:2796282/1‑41 (MQ=255) cccGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTACGTAATGa > 1:929255/1‑67 (MQ=255) cccGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGGTTTGACGCTGAAGTGCTCTTACGTAATGACg < 1:254330/69‑1 (MQ=255) | TGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTACGTAATGACG > W3110S.gb/3347805‑3347938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |