| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,349,295 | C→T | 45.2% | A124T (GCC→ACC) | mtgA ← | biosynthetic peptidoglycan transglycosylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,349,295 | 0 | C | T | 45.2% | 6.4 / 38.4 | 31 | A124T (GCC→ACC) | mtgA | biosynthetic peptidoglycan transglycosylase |
| Reads supporting (aligned to +/- strand): ref base C (10/7); new base T (8/6); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.09e-01 | |||||||||||
AATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCT > W3110S.gb/3349234‑3349362 | aaTCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGttg < 1:1537543/64‑1 (MQ=255)aaTCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGttg < 1:2067125/64‑1 (MQ=255)aaTCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGctgtctg > 1:3275878/1‑68 (MQ=255)aaTCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGctg > 1:2170724/1‑64 (MQ=255) cGGCTTCCAGCCCTTTTCGCACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGctg > 1:61313/1‑60 (MQ=255) gCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGaa > 1:121987/1‑69 (MQ=255) gCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGaa > 1:651516/1‑69 (MQ=255) gCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGaa > 1:1773995/1‑69 (MQ=255) ttCGAACCCATCTACGCCCATCCCATAAAAAGAGATTTTTGGttg > 1:1816014/1‑45 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGctg < 1:2689203/45‑1 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGctg < 1:2742569/45‑1 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGctg < 1:1821583/45‑1 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGctg < 1:1803178/45‑1 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGc > 1:228541/1‑67 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTgaga < 1:1801445/55‑1 (MQ=255) ttCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAg > 1:2194807/1‑66 (MQ=255) cccAGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGaa < 1:90415/69‑1 (MQ=255) aGCTACGCCCATCCCATAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATAc < 1:3042723/69‑1 (MQ=255) tACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGt > 1:2578965/1‑69 (MQ=255) aCGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGtt > 1:2231621/1‑69 (MQ=255) aCGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGtt > 1:2961329/1‑69 (MQ=255) cccatcccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGtttt < 1:1166052/68‑1 (MQ=255) ccatAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCg > 1:1246847/1‑38 (MQ=255) ccatAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTgcg > 1:3423828/1‑69 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAAt < 1:2031164/36‑1 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAAt < 1:295820/36‑1 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTg > 1:199453/1‑41 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGt > 1:1057728/1‑59 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTgcg > 1:3287547/1‑69 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTgcg > 1:2541098/1‑69 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTgcg > 1:2597875/1‑69 (MQ=255) ccatAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTgcg > 1:760180/1‑69 (MQ=255) tAAAAAGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTc < 1:1943906/69‑1 (MQ=255) aaaaGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCa > 1:2423802/1‑59 (MQ=255) aaaaGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCa > 1:2703492/1‑59 (MQ=255) aaaaGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGt < 1:2886276/69‑1 (MQ=255) aaaaGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGt < 1:2994667/69‑1 (MQ=255) aaaaGAGATTTTTGGTTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGt < 1:3127298/69‑1 (MQ=255) agaTTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTgc < 1:2992710/69‑1 (MQ=255) aTTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTgcgc < 1:2372243/69‑1 (MQ=255) aTTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTgcgc < 1:2570096/69‑1 (MQ=255) ctgtctgtTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCt < 1:3156291/68‑1 (MQ=255) | AATCCGGCTTCCAGCCCTTTTCGAACCCAGCTACGCCCATCCCATAAAAAGAGATTTTTGGCTGTCTGTTGAGAAATCGTTGAAGCACCGCGAATACGGTTTTCATTGCGCTCGTTGTGCGCCAGGGCT > W3110S.gb/3349234‑3349362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |