| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,358,271 | A→G | 72.7% | K1262R (AAA→AGA) | gltB → | glutamate synthase, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,358,271 | 0 | A | G | 72.7% | 53.4 / 20.3 | 33 | K1262R (AAA→AGA) | gltB | glutamate synthase, large subunit |
| Reads supporting (aligned to +/- strand): ref base A (3/6); new base G (11/13); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.98e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCG > W3110S.gb/3358207‑3358336 | aaCGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAcc < 1:2279693/69‑1 (MQ=255)aaCGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAcc < 1:914956/69‑1 (MQ=255) gAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGa > 1:1371091/1‑69 (MQ=255) gAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGa > 1:3696637/1‑69 (MQ=255) aaCGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAAc < 1:966823/56‑1 (MQ=255) aaCGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAAc < 1:2403041/56‑1 (MQ=255) aaCGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGat > 1:173988/1‑69 (MQ=255) gTTGCTGCAACAGTCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCaa < 1:871065/69‑1 (MQ=255) gTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCaa < 1:142080/69‑1 (MQ=255) gTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCaa > 1:219701/1‑69 (MQ=255) ttGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAac < 1:230478/69‑1 (MQ=255) ttGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAac < 1:2633523/69‑1 (MQ=255) tgctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGat > 1:1190603/1‑59 (MQ=255) tgctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAaca > 1:540856/1‑69 (MQ=255) tgctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAac > 1:1927361/1‑68 (MQ=255) gctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGa < 1:3114027/57‑1 (MQ=255) gctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGCAaca > 1:975680/1‑68 (MQ=255) ctgcAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCg < 1:1242331/55‑1 (MQ=255) aCAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccgaccg < 1:1832968/69‑1 (MQ=255) aCAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACaccgaccg < 1:1722204/69‑1 (MQ=255) gCGAAACCGTTTGTCGATGAGCGCCAGAGCAGAACCTTc > 1:1995671/1‑39 (MQ=255) tttGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGgcgc < 1:1033695/68‑1 (MQ=255) tGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGa > 1:3515211/1‑36 (MQ=255) tGTCGATGAGCGCCAGAGCAGAACCTTCTGGTTCGa > 1:1228001/1‑36 (MQ=255) gATGAGCGCCAGAGCAGAACCTTCTGGTTCGATATTCGc > 1:2602520/1‑39 (MQ=255) cgcCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc < 1:1984946/69‑1 (MQ=255) cgcCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc < 1:3236234/69‑1 (MQ=255) cgcCAGAGCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGc < 1:528527/69‑1 (MQ=255) agagCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCta < 1:1795221/67‑1 (MQ=255) agagCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCta < 1:3722427/67‑1 (MQ=255) agagCAGAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCta < 1:751155/67‑1 (MQ=255) cAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCg > 1:2297799/1‑68 (MQ=255) cAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCg > 1:166706/1‑68 (MQ=255) | AACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATATTCGCAACACCGACCGTTCTGTCGGCGCGTCGCTTTCAGGCTATATCG > W3110S.gb/3358207‑3358336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |