| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,365,218 | A→G | 62.8% | pseudogene (179/480 nt) pseudogene (179/1745 nt) |
yhcE → yhcE → |
ECK3207:JW3184:b3217; hypothetical protein, N‑ter fragment ECK3207:JW3184+JW3187:b4569; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,365,218 | 0 | A | G | 62.8% | 90.5 / 100.6 | 94 | pseudogene (179/480 nt) pseudogene (179/1745 nt) | yhcE yhcE | ECK3207:JW3184:b3217; hypothetical protein, N‑ter fragment ECK3207:JW3184+JW3187:b4569; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (14/21); new base G (21/38); total (35/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
Only 100 of 116 total aligned reads displayed.
CGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGA > W3110S.gb/3365157‑3365285 | cgcCAAGTTGACCTGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAatgcgatgcg > 1:1890127/1‑67 (MQ=255)cgcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAatgcgatgcg > 1:3314536/1‑67 (MQ=255)cgcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAatgcgatgcg > 1:2600356/1‑67 (MQ=255)cgcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAatgcgatgcg > 1:3668746/1‑67 (MQ=255)cgcCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGAt < 1:1918265/69‑1 (MQ=255) cAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAg < 1:1983038/69‑1 (MQ=255) tGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACg > 1:3053628/1‑68 (MQ=255) cGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCa < 1:1709016/68‑1 (MQ=255) ggTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATAtt < 1:3677117/69‑1 (MQ=255) ggTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATAtt < 1:369747/69‑1 (MQ=255) ggTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATAtt < 1:2028826/69‑1 (MQ=255) ggTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATAtt < 1:3672860/69‑1 (MQ=255) ggTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATAtt < 1:2561230/69‑1 (MQ=255) gTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTGATGCGATGCGATCAGGACGCTCATATTc < 1:3241406/69‑1 (MQ=255) gTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTc < 1:2015869/69‑1 (MQ=255) tAAAACAACACAATTAGCATACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCg < 1:1936756/69‑1 (MQ=255) tAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCg < 1:911623/69‑1 (MQ=255) aaaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCgt > 1:708868/1‑69 (MQ=255) aaaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGAACGCTCATATTCgt > 1:3349383/1‑69 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCgtg < 1:1570718/69‑1 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCgtg > 1:544075/1‑69 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCgtg > 1:1782142/1‑69 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCgtg > 1:1411646/1‑69 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCgt > 1:3241232/1‑68 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCgtg > 1:2852809/1‑69 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCgtg > 1:694532/1‑69 (MQ=255) aaaCAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCgtg > 1:1049109/1‑69 (MQ=255) aacaacACAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCgtg < 1:1788798/68‑1 (MQ=255) caacaCAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTc < 1:3555353/69‑1 (MQ=255) caacaCAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTc < 1:1974721/69‑1 (MQ=255) caacaCAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTc < 1:1711892/69‑1 (MQ=255) acacAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAg < 1:2026906/69‑1 (MQ=255) acacAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAg < 1:1058306/69‑1 (MQ=255) acacAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAg < 1:814929/69‑1 (MQ=255) acacAATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAg < 1:3654584/69‑1 (MQ=255) caATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:1422470/69‑1 (MQ=255) caATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:3369549/69‑1 (MQ=255) caATTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:3478993/69‑1 (MQ=255) caATTAGCAGACAGAACAATCACGCTGCTAATGCGATGCGATCAGGACGCCCATATTCGTGTTCAGTTg < 1:866422/69‑1 (MQ=255) aaTTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg > 1:1032277/1‑68 (MQ=255) aaTTAGCAGACAGAACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg > 1:1306619/1‑68 (MQ=255) ttAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:1022896/66‑1 (MQ=255) ttAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:1375596/66‑1 (MQ=255) ttAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:2279146/66‑1 (MQ=255) aGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg > 1:2980727/1‑64 (MQ=255) aGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTg > 1:2976383/1‑64 (MQ=255) aGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATAc > 1:2514529/1‑69 (MQ=255) gacggaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt < 1:712805/69‑1 (MQ=255) gacagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt < 1:1576073/69‑1 (MQ=255) gacagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt < 1:2873589/69‑1 (MQ=255) cagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:1288318/58‑1 (MQ=255) cagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTaa < 1:2497042/69‑1 (MQ=255) cagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTaa > 1:2227476/1‑69 (MQ=255) cagaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTaa < 1:347888/69‑1 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCa < 1:3663801/53‑1 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCa < 1:2613199/53‑1 (MQ=255) agaACAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCa < 1:355917/53‑1 (MQ=255) agaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCata < 1:2746488/43‑1 (MQ=255) agaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCata < 1:892653/43‑1 (MQ=255) agaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCa > 1:65077/1‑41 (MQ=255) agaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCa > 1:93770/1‑41 (MQ=255) agaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCa > 1:302335/1‑41 (MQ=255) agaACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCa > 1:1226039/1‑41 (MQ=255) aCAATCACGCTGGTAATGGGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGaa > 1:2276896/1‑56 (MQ=255) aCAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGaa > 1:874276/1‑56 (MQ=255) aCAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGaa > 1:1704926/1‑56 (MQ=255) aCAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGaa > 1:2851448/1‑56 (MQ=255) aCAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGaa > 1:175839/1‑56 (MQ=255) aCAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTattatt > 1:3228947/1‑69 (MQ=255) cAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:628588/53‑1 (MQ=255) cAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:3351606/53‑1 (MQ=255) cAATCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTg < 1:194174/53‑1 (MQ=255) aTCACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTcc > 1:463350/1‑69 (MQ=255) aTCACGCTGGTAAAGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTcc > 1:589311/1‑69 (MQ=255) cACGCTTGTAATGCGATGCGATCAGTACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCca > 1:2429924/1‑68 (MQ=255) cACGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCca > 1:267736/1‑68 (MQ=255) aCGCTGGTAATGCGATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCcaac > 1:1024691/1‑69 (MQ=255) aCGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCcaac < 1:3411404/69‑1 (MQ=255) aCGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCcaac < 1:2432199/69‑1 (MQ=255) cGCTGGTAATGCGATGCGATCAGGACGCTCATAtt < 1:1244970/35‑1 (MQ=255) tAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGt < 1:2318974/39‑1 (MQ=255) tAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGt < 1:2445870/39‑1 (MQ=255) tAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGt < 1:1934309/39‑1 (MQ=255) tAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGt < 1:1281388/39‑1 (MQ=255) aatgcgatgcGATCAGTACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACgga < 1:1567160/67‑1 (MQ=255) aatgcgatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACgga < 1:470767/67‑1 (MQ=255) aatgcgatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACgga < 1:2621690/67‑1 (MQ=255) aatgcgatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACgga < 1:61732/67‑1 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAat > 1:2894533/1‑51 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAat > 1:1045864/1‑51 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAat > 1:2903569/1‑51 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAat > 1:3638465/1‑51 (MQ=255) atgcgatgcgATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACggatt > 1:2496326/1‑68 (MQ=255) caatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt > 1:2868237/1‑45 (MQ=255) caatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCt > 1:1057349/1‑45 (MQ=255) caatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAata > 1:3148193/1‑49 (MQ=255) caatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAata > 1:3119099/1‑49 (MQ=255) caatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACggatttgga > 1:1300799/1‑69 (MQ=255) caatgcGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACggatttgga > 1:656521/1‑69 (MQ=255) caatgcGATCAGGACGCTCATATTAGTGTTCAGTTGAATACCGCTAata > 1:715911/1‑49 (MQ=255) | CGCCAAGTTGACCGGGGTAAAACAACACAATTAGCAGACAGAACAATCACGCTGGTAATGCAATGCGATCAGGACGCTCATATTCGTGTTCAGTTGAATACCGCTAATATTTCCAACAACGGATTTGGA > W3110S.gb/3365157‑3365285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |