| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,398,077 | C→T | 22.7% | A53T (GCA→ACA) | yhdE ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,398,077 | 0 | C | T | 22.7% | 40.3 / 8.9 | 22 | A53T (GCA→ACA) | yhdE | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (7/10); new base T (3/2); total (10/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCACAACATACTGCTGCGCGCTCTCCTGCGGCTGACGCTGCTCCTCAATGC > W3110S.gb/3398016‑3398145 | aCGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGTTTTCTcg > 1:1483828/1‑69 (MQ=255)aCGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGTTTTCTcg > 1:2180114/1‑69 (MQ=255)aCGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGTTTTATcg > 1:1412490/1‑69 (MQ=255) gTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCgcgcgc < 1:3004168/68‑1 (MQ=255) tCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGa > 1:1876868/1‑69 (MQ=255) cccAGCACCGGGAGATCCTTCGCCGTTTGGGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGcac > 1:1198140/1‑68 (MQ=255) cccAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGcac > 1:1828098/1‑68 (MQ=255) aGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGTTTTCTCGCGCGCCAGACGCacaaca < 1:2828088/69‑1 (MQ=255) aGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaaca < 1:1364237/69‑1 (MQ=255) aGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaaca < 1:648791/69‑1 (MQ=255) aGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaaca > 1:597064/1‑69 (MQ=255) aGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaaca < 1:319363/69‑1 (MQ=255) aGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaaca < 1:2900511/69‑1 (MQ=255) gCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGTTTTCTCGCGCGCCAGACGCacaaca < 1:2240630/68‑1 (MQ=255) cACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCACAACAt > 1:2754073/1‑68 (MQ=255) gaTCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaa < 1:3085326/57‑1 (MQ=255) gaTCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaa < 1:1416034/57‑1 (MQ=255) gaTCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCacaa < 1:884622/57‑1 (MQ=255) ccTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCACAACATACTGCTgcgcgc < 1:1556554/69‑1 (MQ=255) ttCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCACAACATACTGCTgcg > 1:1676964/1‑64 (MQ=255) ttCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCACAACATACTGCTgcg > 1:1372369/1‑64 (MQ=255) cGTGCTTTCTCGCGCGCCAGACGCACAACATACTGCTGCGCGCTCTCCTGCGGCTGACGCTGctcct < 1:2343330/67‑1 (MQ=255) cTTTCTCGCGCGCCAGACGCACAACATACTGCTGCGCGCTCTCCTGCGGCTGACGCTGctc < 1:1388511/61‑1 (MQ=255) cTTTCTCGCGCGCCAGACGCACAACATACTGCTGCGCGCTCTCCTGCGGCTGACGCTGCTCCTCAATGc > 1:2681995/1‑69 (MQ=255) cTTTATCGCGCGCCAGACGCACAACATACTGCTGCGCGct > 1:3307192/1‑40 (MQ=255) | ACGATAGTATCCGCACCCAGCACCGGGAGATCCTTCGCCGTTTGCGCGACACCTGCCCGTGCTTTCTCGCGCGCCAGACGCACAACATACTGCTGCGCGCTCTCCTGCGGCTGACGCTGCTCCTCAATGC > W3110S.gb/3398016‑3398145 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |