| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 234,234 | C→T | 65.1% | L183L (TTG→TTA) | gloB ← | predicted hydroxyacylglutathione hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 234,234 | 0 | C | T | 65.1% | 62.5 / 62.3 | 63 | L183L (TTG→TTA) | gloB | predicted hydroxyacylglutathione hydrolase |
| Reads supporting (aligned to +/- strand): ref base C (11/11); new base T (24/17); total (35/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA > W3110S.gb/234168‑234297 | tACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATaa < 1:1410496/69‑1 (MQ=255) gTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGt > 1:3395756/1‑69 (MQ=255) gTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGt > 1:1429100/1‑69 (MQ=255) tgtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGc > 1:972350/1‑67 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCgg < 1:1355240/68‑1 (MQ=255) gtTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCgg < 1:1466602/68‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGga > 1:1092604/1‑68 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGga > 1:740/1‑68 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGga > 1:3107525/1‑68 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGga > 1:2987457/1‑68 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGga > 1:274283/1‑68 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGga > 1:2414797/1‑68 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag < 1:2848828/68‑1 (MQ=255) aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaag < 1:3418698/68‑1 (MQ=255) ttGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa < 1:840992/68‑1 (MQ=255) ttGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa < 1:586509/68‑1 (MQ=255) ttGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGgaagaa < 1:691562/68‑1 (MQ=255) ttttGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCaa > 1:1403587/1‑69 (MQ=255) ttttGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACa > 1:2177593/1‑47 (MQ=255) ttttGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACa > 1:1378625/1‑47 (MQ=255) ttttGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACa > 1:1602307/1‑47 (MQ=255) ccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCaa < 1:179754/68‑1 (MQ=255) ccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCaa < 1:535920/68‑1 (MQ=255) ccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCaa < 1:1197045/68‑1 (MQ=255) ccGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCaa < 1:2396830/68‑1 (MQ=255) ccGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCaa < 1:2401223/68‑1 (MQ=255) cGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCaa < 1:241252/67‑1 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCa < 1:21045/62‑1 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCa > 1:1892005/1‑69 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCa > 1:813524/1‑69 (MQ=255) aCTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCa > 1:3297708/1‑69 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATaa > 1:339511/1‑35 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATaa > 1:929681/1‑35 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATaa > 1:2562074/1‑35 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaaga > 1:1436238/1‑48 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaaga > 1:570029/1‑48 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata < 1:1223256/69‑1 (MQ=255) tCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCCAACTTCa < 1:2599246/67‑1 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATAc < 1:1275675/47‑1 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATAc > 1:2349863/1‑47 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATAc < 1:2008565/47‑1 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATAttt > 1:2392435/1‑67 (MQ=255) aaCTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTg > 1:28424/1‑68 (MQ=255) aaCTTTACGATAATAATCATTTATGGACAAATCGTGCGgaag > 1:3472507/1‑42 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAgg > 1:3323931/1‑69 (MQ=255) tACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAgg > 1:3254031/1‑69 (MQ=255) cGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGt < 1:707068/68‑1 (MQ=255) gATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:3375088/68‑1 (MQ=255) gATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:903915/68‑1 (MQ=255) gATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta < 1:2238351/68‑1 (MQ=255) taataaTCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata < 1:832436/54‑1 (MQ=255) taataaTCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata > 1:139299/1‑54 (MQ=255) taataaTCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata < 1:2569457/54‑1 (MQ=255) aataatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTatat > 1:3375651/1‑68 (MQ=255) taatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt < 1:1133619/67‑1 (MQ=255) taatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt > 1:3352416/1‑67 (MQ=255) taatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATAtt > 1:1315345/1‑67 (MQ=255) aatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCa < 1:2690610/68‑1 (MQ=255) atCATTTATGGATAAATCGTGCGGTAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTc > 1:3583649/1‑66 (MQ=255) cATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAg > 1:712319/1‑69 (MQ=255) cATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAg > 1:1497428/1‑69 (MQ=255) tATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcac > 1:3096499/1‑68 (MQ=255) tGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGTTATATTCATGAGCacaa < 1:423260/68‑1 (MQ=255) | TACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA > W3110S.gb/234168‑234297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |