| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,447,843 | A→G | 77.5% | V1163V (GTT→GTC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,447,843 | 0 | A | G | 77.5% | 80.6 / 19.4 | 40 | V1163V (GTT→GTC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base G (12/19); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAAC > W3110S.gb/3447779‑3447900 | gATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGa < 1:539765/69‑1 (MQ=255) gCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGa < 1:2548082/65‑1 (MQ=255) gCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGa > 1:1516592/1‑65 (MQ=255) gTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGa < 1:3213289/68‑1 (MQ=255) gTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGa < 1:2391102/68‑1 (MQ=255) gTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt > 1:15359/1‑69 (MQ=255) gTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGAt > 1:431037/1‑69 (MQ=255) tCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGa < 1:2469705/67‑1 (MQ=255) tCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGa < 1:2204722/67‑1 (MQ=255) ccGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc < 1:2813932/52‑1 (MQ=255) ccGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc < 1:157098/52‑1 (MQ=255) ccGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGAc < 1:1643833/52‑1 (MQ=255) ccGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCa > 1:3558308/1‑68 (MQ=255) ggggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGa > 1:857475/1‑52 (MQ=255) ggggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGcc > 1:3123578/1‑69 (MQ=255) ggTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAg < 1:2098085/69‑1 (MQ=255) gTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:1053854/68‑1 (MQ=255) aTAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCt > 1:1869084/1‑53 (MQ=255) aaCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCa > 1:1101653/1‑58 (MQ=255) aCCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGcc < 1:315811/69‑1 (MQ=255) aGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGc > 1:1162230/1‑69 (MQ=255) acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGcag > 1:210791/1‑54 (MQ=255) acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:225930/1‑57 (MQ=255) acgacgTTTACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:854992/1‑57 (MQ=255) cgTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCttt > 1:3048024/1‑68 (MQ=255) gTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCg < 1:2048273/69‑1 (MQ=255) gTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCg < 1:1630963/69‑1 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgac < 1:2373179/69‑1 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgac < 1:3709876/69‑1 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgac > 1:3165641/1‑69 (MQ=255) tACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGa > 1:1968473/1‑51 (MQ=255) cTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga < 1:1001172/68‑1 (MQ=255) cTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga < 1:2132979/68‑1 (MQ=255) cTTTGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGgacga < 1:1411696/68‑1 (MQ=255) tttGGTTTCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGacgacg < 1:984406/69‑1 (MQ=255) ttCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:3163766/39‑1 (MQ=255) ttCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:2324266/39‑1 (MQ=255) ttCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg < 1:830171/39‑1 (MQ=255) tCTTTACCGAAGGAGACGATACCGCTGATTTCAGCCAg > 1:362768/1‑38 (MQ=255) ccGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAAc < 1:1183900/66‑1 (MQ=255) | GATCGCTACCGTCTACCGGGGTGATAACCAGACGACGTTTACCTTTGGTTTCTTTACCGAAGGAAACGATACCGCTGATTTCAGCCAGGATTGCCGGCTCTTTCGGACGACGTGCTTCGAAC > W3110S.gb/3447779‑3447900 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |