| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,567,710 | T→C | 35.0% | R102R (CGT→CGC) | yihQ → | alpha‑glucosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,567,710 | 0 | T | C | 35.0% | 14.9 / 16.5 | 20 | R102R (CGT→CGC) | yihQ | alpha‑glucosidase |
| Reads supporting (aligned to +/- strand): ref base T (8/5); new base C (4/3); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCA > W3110S.gb/3567651‑3567775 | ttCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGtt < 1:1827303/61‑1 (MQ=255) tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTgg > 1:807165/1‑69 (MQ=255) tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTgg > 1:3565610/1‑69 (MQ=255) tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTgg > 1:3411860/1‑69 (MQ=255) tCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTg > 1:3191191/1‑68 (MQ=255) tCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAAc < 1:19042/69‑1 (MQ=255) tCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAAc < 1:23465/69‑1 (MQ=255) cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGtt < 1:1589722/48‑1 (MQ=255) cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGtt > 1:1600657/1‑48 (MQ=255) cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGtt > 1:3453866/1‑48 (MQ=255) cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAAc > 1:1311267/1‑68 (MQ=255) cTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAAc > 1:2789333/1‑68 (MQ=255) aTTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACaa < 1:3429579/68‑1 (MQ=255) aGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCtt < 1:2209319/69‑1 (MQ=255) tatCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCtt < 1:3312738/55‑1 (MQ=255) tatCTCTGCCGACGATCAGGGGCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAAGCGt < 1:2227041/67‑1 (MQ=255) tctcTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAAc > 1:28821/1‑50 (MQ=255) tctcTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAAc > 1:1848970/1‑50 (MQ=255) tctGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTg < 1:3232321/68‑1 (MQ=255) cAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGcc > 1:988929/1‑69 (MQ=255) cAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGc > 1:2689719/1‑68 (MQ=255) ggCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCt > 1:1234312/1‑68 (MQ=255) ggCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTc < 1:2673722/69‑1 (MQ=255) ggCGCTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTc > 1:67390/1‑69 (MQ=255) tttGTTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCa > 1:989506/1‑66 (MQ=255) | TTCAGCCGTGGTTCTGACATTAGCGCCACGCTGAATATCTCTGCCGACGATCAGGGGCGTTTATTGCTGGAACTACAAAACGACAACCTTAACCACAACCGTATCTGGCTGCGCCTTGCCGCTCA > W3110S.gb/3567651‑3567775 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |