| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,587,198 | C→T | 83.9% | E840K (GAA→AAA) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,587,198 | 0 | C | T | 83.9% | 71.1 / 8.3 | 31 | E840K (GAA→AAA) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base C (3/2); new base T (12/14); total (15/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TCATCGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACGGCGTCCGTCCA > W3110S.gb/3587135‑3587263 | tcatcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCg > 1:933466/1‑69 (MQ=255)tcatcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCg > 1:898350/1‑69 (MQ=255) atcGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGc > 1:220724/1‑68 (MQ=255) gCCCGTTTGATAATGTCGGCGGCGCTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGc > 1:2107069/1‑68 (MQ=255) ccGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCac < 1:1773101/68‑1 (MQ=255) aTAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGc < 1:3148596/68‑1 (MQ=255) aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCAc < 1:1910194/68‑1 (MQ=255) tGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCAcc > 1:3530367/1‑67 (MQ=255) gTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAtt > 1:695305/1‑69 (MQ=255) gTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAtt > 1:1745268/1‑69 (MQ=255) gTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAt > 1:1263844/1‑68 (MQ=255) gTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAt > 1:197000/1‑68 (MQ=255) tCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTg > 1:3097491/1‑69 (MQ=255) tCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTg > 1:2352748/1‑69 (MQ=255) gcggTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCAc > 1:443676/1‑58 (MQ=255) gTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTg < 1:1943381/69‑1 (MQ=255) gTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTg < 1:1492261/69‑1 (MQ=255) tCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGat < 1:1064538/69‑1 (MQ=255) tGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCa > 1:413267/1‑68 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTg < 1:2644392/51‑1 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTg < 1:3565451/51‑1 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTg < 1:102587/51‑1 (MQ=255) cgTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:827060/69‑1 (MQ=255) aaTGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGAc < 1:3049240/68‑1 (MQ=255) aCGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACGGcgtccg > 1:1292375/1‑67 (MQ=255) cGTTTAGCCGCTGCACGACGAGCACCATTGCTGGAttt < 1:2931972/38‑1 (MQ=255) cGTTTAGCCGCTGCACGACGAGCACCATTGCTGGAttt < 1:2248125/38‑1 (MQ=255) cGTTTAGCCGCTGCACGACGAGCACCATTGCTGGAttt < 1:1422759/38‑1 (MQ=255) cGTTTAGCCGCTGCACGACGAGCACCATTGCTGGAttt < 1:861376/38‑1 (MQ=255) cGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACGGcgtccgt < 1:3550688/67‑1 (MQ=255) gTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACGGCGTCCGTCCa > 1:3435104/1‑69 (MQ=255) | TCATCGCCCGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACGGCGTCCGTCCA > W3110S.gb/3587135‑3587263 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |