| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,634,018 | T→C | 11.3% | V46A (GTA→GCA) | yigF → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,634,018 | 0 | T | C | 11.3% | 139.9 / 8.9 | 53 | V46A (GTA→GCA) | yigF | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (32/15); new base C (4/2); total (36/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTGTCC > W3110S.gb/3633951‑3634086 | cAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGt < 1:1753491/68‑1 (MQ=255) aTGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGAc < 1:834152/69‑1 (MQ=255) ggTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACaa > 1:2298662/1‑69 (MQ=255) gTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGCAAGACAAc > 1:2268275/1‑69 (MQ=255) ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGc > 1:2921087/1‑68 (MQ=255) ttCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGc > 1:1993475/1‑68 (MQ=255) ccTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCtt < 1:3231769/68‑1 (MQ=255) ggATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACt > 1:2466079/1‑69 (MQ=255) gATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACt > 1:221794/1‑68 (MQ=255) gggggCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCa > 1:2334989/1‑66 (MQ=255) gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAAt > 1:991948/1‑69 (MQ=255) gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAAt < 1:3049252/69‑1 (MQ=255) gCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAAt < 1:719094/69‑1 (MQ=255) acacCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAAttt < 1:2492689/68‑1 (MQ=255) acCAGAATATAAAGCAGGATTTAAAGCATTAAAAGTAAGAc > 1:2744309/1‑41 (MQ=255) acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGAc > 1:3123903/1‑41 (MQ=255) acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGAc > 1:3448817/1‑41 (MQ=255) acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCa > 1:1686023/1‑68 (MQ=255) acCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCAt > 1:3094556/1‑69 (MQ=255) ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGt < 1:212360/35‑1 (MQ=255) ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGt < 1:210631/35‑1 (MQ=255) ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGt < 1:1899777/35‑1 (MQ=255) ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGt < 1:3623346/35‑1 (MQ=255) ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGt < 1:1060975/35‑1 (MQ=255) ccAGAATATAAAGCAGCATTTAAAGCATTAAAAGt < 1:87903/35‑1 (MQ=255) ccAGAATATAAAGCACCATTTAAAGCATTAAAAGt < 1:3573424/35‑1 (MQ=255) taAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttctt > 1:2218215/1‑69 (MQ=255) taAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttctt > 1:1843903/1‑69 (MQ=255) taAAGCAGCATTTAAAGCATTAAAAGTAAGACAAAGCTTAACTATTCAAATGAATTTCATTGCTttctt > 1:1975673/1‑69 (MQ=255) taAAGCAGCATTTAAAGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttctt > 1:524360/1‑69 (MQ=255) aaGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGc > 1:2433051/1‑61 (MQ=255) aaGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttcttc < 1:2545359/68‑1 (MQ=255) aaGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttcttc < 1:2887657/68‑1 (MQ=255) aaGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTtcttct > 1:3038456/1‑69 (MQ=255) agcagcATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttcttc < 1:3007069/67‑1 (MQ=255) gcagcaTTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:2806232/69‑1 (MQ=255) gcagcaTTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGt < 1:2115610/69‑1 (MQ=255) aTTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTg < 1:2472029/69‑1 (MQ=255) aTTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTg > 1:1654652/1‑69 (MQ=255) aTTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTg < 1:2145256/69‑1 (MQ=255) tttAAAGCATTAAAAGTAAGACAAGGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTgg > 1:1742896/1‑69 (MQ=255) tttAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTg > 1:945045/1‑68 (MQ=255) ttAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa < 1:3423807/69‑1 (MQ=255) tAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa > 1:314659/1‑68 (MQ=255) aGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGatat < 1:3488030/68‑1 (MQ=255) aGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGatat < 1:1553605/68‑1 (MQ=255) aGCATTAAAAGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGatat < 1:2605909/68‑1 (MQ=255) cATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatt < 1:3551455/69‑1 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTttcttc > 1:414806/1‑49 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTc > 1:2398735/1‑54 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGa > 1:3489790/1‑59 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatttatt > 1:1857385/1‑69 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatttatt > 1:3359211/1‑69 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatttatt > 1:1682181/1‑69 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatttatt > 1:3136767/1‑69 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatttatt > 1:932798/1‑69 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatttatt > 1:304178/1‑69 (MQ=255) aaaaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATAtatttat > 1:3572987/1‑68 (MQ=255) aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTg > 1:1656989/1‑41 (MQ=255) aaaaGCAAGACAACGCTTAACTATTCAAATGAATTTCATTGCt > 1:2847713/1‑43 (MQ=255) aaGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTg > 1:3140563/1‑69 (MQ=255) tAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTGTcc > 1:2465411/1‑69 (MQ=255) | CAACATGGTTTTCCTGGATTCTGGGGGGCAACACCAGAATATAAAGCAGCATTTAAAGCATTAAAAGTAAGACAACGCTTAACTATTCAAATGAATTTCATTGCTTTCTTCTGTTCCTGGATATATTTATTTGTCC > W3110S.gb/3633951‑3634086 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |