| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,705,799 | A→G | 50.0% | E70E (GAA→GAG) | yieN → | fused predicted transcriptional regulators |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,705,799 | 0 | A | G | 50.0% | ‑5.6 / 19.5 | 16 | E70E (GAA→GAG) | yieN | fused predicted transcriptional regulators |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base G (2/6); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCAGGTATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGGC > W3110S.gb/3705731‑3705858 | ccAGGTATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGaa < 1:2894909/69‑1 (MQ=255) cAGGTATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAat < 1:1853666/69‑1 (MQ=255) aTTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCtg > 1:3552248/1‑69 (MQ=255) aTTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCtg > 1:983841/1‑69 (MQ=255) aTTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCtg > 1:654187/1‑69 (MQ=255) ttGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCtg > 1:3406364/1‑68 (MQ=255) aaaGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCTGATGAccc < 1:1276244/69‑1 (MQ=255) tGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCTGATGACCCGCTtct < 1:221915/69‑1 (MQ=255) aTTCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGtttt < 1:1684487/69‑1 (MQ=255) ttCGCCTTTCAGAATGCCCGCGCGTTTGAGTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGtttt < 1:1619416/68‑1 (MQ=255) ccTTTCAGAATGCCCGCGCGTTTGAGTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGt < 1:2780292/68‑1 (MQ=255) aaTGCCCGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTc < 1:796916/61‑1 (MQ=255) gCCCGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTAt < 1:1286336/68‑1 (MQ=255) cccGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTc > 1:2977372/1‑69 (MQ=255) cccGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTc > 1:2777420/1‑69 (MQ=255) cccGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTc > 1:3664183/1‑69 (MQ=255) cgcgTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGgc > 1:1599271/1‑69 (MQ=255) tGAGTATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTcccc < 1:1336767/50‑1 (MQ=255) | CCAGGTATTGCCAAAAGTTTGATCGCCCGGCGCTTAAAATTCGCCTTTCAGAATGCCCGCGCGTTTGAATATCTGATGACCCGCTTCTCCACGCCGGAAGAAGTTTTTGGTCCCCTTTCTATTCAGGC > W3110S.gb/3705731‑3705858 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |