Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,709,115	C→T	17.1%	E138E (GAG→GAA)	asnA ←	asparagine synthetase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,709,115	0	C	T	17.1%	100.4 / 13.0	41	E138E (GAG→GAA)	asnA	asparagine synthetase A
Reads supporting (aligned to +/- strand):  ref base C (22/12);  new base T (3/4);  total (25/16)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCAC  >  W3110S.gb/3709048‑3709181                                                                    |                                                                   ggAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGcc                                                                    <  1:2479936/68‑1 (MQ=255)   aaCGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCctct                                                                 <  1:2727125/69‑1 (MQ=255)   aaCGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCctct                                                                 <  1:1350141/69‑1 (MQ=255)   aaCGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCctct                                                                 >  1:410953/1‑69 (MQ=255)   aaCGGTGCCAGGCCAAACTCTCCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCctct                                                                 >  1:2014038/1‑69 (MQ=255)    aCGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCctct                                                                 >  1:1139637/1‑68 (MQ=255)    aCGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTa                                                                >  1:1092624/1‑69 (MQ=255)    aCGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTa                                                                >  1:321861/1‑69 (MQ=255)    aCGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCAAGATCGCCTCTa                                                                >  1:3386458/1‑69 (MQ=255)         gCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTg                                                           >  1:7167/1‑69 (MQ=255)            aGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtt                                                        <  1:2227325/69‑1 (MQ=255)            aGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtt                                                        <  1:2944614/69‑1 (MQ=255)               ccAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtt                                                        >  1:1756175/1‑66 (MQ=255)                cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtttt                                                      >  1:958541/1‑67 (MQ=255)                cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtttt                                                      >  1:2823333/1‑67 (MQ=255)                cAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCa                                                    <  1:367633/69‑1 (MQ=255)                    ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCtt                                                        <  1:962905/61‑1 (MQ=255)                    ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCagag                                                 >  1:1978028/1‑68 (MQ=255)                    ctctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCagag                                                 >  1:2752143/1‑68 (MQ=255)                      ctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCagag                                                 >  1:3189672/1‑66 (MQ=255)                      ctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCagag                                                 >  1:2939997/1‑66 (MQ=255)                      ctTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCagag                                                 >  1:3389613/1‑66 (MQ=255)                       ttCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCg                                              >  1:1750133/1‑68 (MQ=255)                       ttCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCTTCTACCGTGCTTTTCAGAGTCg                                              >  1:1130256/1‑68 (MQ=255)                                cGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCaga                                                  <  1:222337/55‑1 (MQ=255)                                cGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCaga                                                  >  1:3654818/1‑55 (MQ=255)                                cGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCaga                                                  <  1:1266235/55‑1 (MQ=255)                                    gCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCa                                <  1:3509460/69‑1 (MQ=255)                                      ttCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAAtt                                        >  1:205553/1‑59 (MQ=255)                                             gCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGccc                       >  1:2622717/1‑69 (MQ=255)                                             gCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGccc                       >  1:2524589/1‑69 (MQ=255)                                             gCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGccc                       >  1:3300098/1‑69 (MQ=255)                                                         gCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc           <  1:2737015/69‑1 (MQ=255)                                                         gCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc           <  1:623233/69‑1 (MQ=255)                                                         gCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc           <  1:2508251/69‑1 (MQ=255)                                                         gCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTcc           <  1:2217812/69‑1 (MQ=255)                                                            cAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTccc          <  1:2767592/67‑1 (MQ=255)                                                            cAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTccc          <  1:1904631/67‑1 (MQ=255)                                                            cAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTccc          <  1:371729/67‑1 (MQ=255)                                                            cAGATCGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTccc          <  1:167362/67‑1 (MQ=255)                                                              gATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGt       >  1:1501934/1‑68 (MQ=255)                                                                tCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTccc    >  1:3382276/1‑69 (MQ=255)                                                                tCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTccc    >  1:1379855/1‑69 (MQ=255)                                                                tCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTccc    >  1:111327/1‑69 (MQ=255)                                                                 cgactctACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCa   >  1:169500/4‑69 (MQ=255)                                                                 cGCTTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCATCGTCGCCCATTACGCGTTCCCAGTCCCa   >  1:3488137/1‑69 (MQ=255)                                                                 cGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCa   >  1:319042/1‑69 (MQ=255)                                                                  gCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCAc  <  1:849614/69‑1 (MQ=255)                                                                    |                                                                   GGAACGGTGCCAGGCCAAACTCTTCGCTAACCGCAGCTTCGGTTGCTTTAATTCCCGCCCAGATCGCCTCTACCGTGCTTTTCAGAGTCGAGAATTGACGCTCACCGTCGCCCATTACGCGTTCCCAGTCCCAC  >  W3110S.gb/3709048‑3709181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑