| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,714,423 | C→T | 48.1% | H61Y (CAC→TAC) | atpI → | ATP synthase, membrane‑bound accesory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,714,423 | 0 | C | T | 48.1% | 5.1 / 76.7 | 54 | H61Y (CAC→TAC) | atpI | ATP synthase, membrane‑bound accesory subunit |
| Reads supporting (aligned to +/- strand): ref base C (19/9); new base T (16/10); total (35/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01 | |||||||||||
TCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGT > W3110S.gb/3714357‑3714490 | tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTc > 1:2781288/1‑67 (MQ=255)tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTc > 1:2776762/1‑67 (MQ=255)tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTc > 1:1986306/1‑67 (MQ=255)tctGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTAc < 1:112159/69‑1 (MQ=255) aaTAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCa > 1:3654310/1‑66 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa < 1:983200/68‑1 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCa < 1:210019/68‑1 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCCCa < 1:1593826/68‑1 (MQ=255) aGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGgc > 1:3020652/1‑65 (MQ=255) cGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATa < 1:3091387/68‑1 (MQ=255) cGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAgg < 1:2971604/62‑1 (MQ=255) ggCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAgg < 1:2600978/58‑1 (MQ=255) ccTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAg > 1:2331826/1‑68 (MQ=255) ccTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAg > 1:3699640/1‑68 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa > 1:1474735/1‑54 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa < 1:2978007/54‑1 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATac > 1:172020/1‑57 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATac > 1:2151720/1‑57 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAgg > 1:1579997/1‑69 (MQ=255) aGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAgg > 1:2413729/1‑69 (MQ=255) gTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa < 1:2656229/53‑1 (MQ=255) tCTTTCTGCTTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg > 1:449888/1‑50 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg < 1:3457404/50‑1 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg < 1:781097/50‑1 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg > 1:950893/1‑50 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg > 1:2599921/1‑50 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg > 1:2358813/1‑50 (MQ=255) tCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg < 1:1084119/50‑1 (MQ=255) acTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGgcg > 1:2085742/2‑50 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa < 1:338071/51‑1 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa < 1:1252010/51‑1 (MQ=255) cTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCa < 1:2563638/51‑1 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTc > 1:1172798/1‑40 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCgg > 1:2246889/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCgg > 1:2152019/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCgg > 1:3551173/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCg > 1:3135387/1‑68 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCgg > 1:1419676/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCgg > 1:3377650/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCgg > 1:1526335/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCgg > 1:3410672/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCgg > 1:1144937/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCgg > 1:3577427/1‑69 (MQ=255) tttCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCgg > 1:1146388/1‑69 (MQ=255) tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCg > 1:1225304/1‑66 (MQ=255) tCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGt > 1:1674014/1‑69 (MQ=255) cTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGcc > 1:64488/1‑68 (MQ=255) aCGTTTTGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGa > 1:1171348/1‑69 (MQ=255) ttttGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCg > 1:1614871/1‑55 (MQ=255) ttttGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCg > 1:789441/1‑55 (MQ=255) ttGTTTATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGaa < 1:343165/47‑1 (MQ=255) tATGATATTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCAtt > 1:1889175/1‑69 (MQ=255) atatTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTgg > 1:1422639/1‑68 (MQ=255) atatTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTgg > 1:1304388/1‑68 (MQ=255) atatTTGCCTGGCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTgg > 1:2735231/1‑68 (MQ=255) ggCGTTACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCa < 1:2303719/69‑1 (MQ=255) ggCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCa < 1:1700428/69‑1 (MQ=255) tCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGt < 1:403140/69‑1 (MQ=255) | TCTGCAATAAGCGGGGGCCTGGCAGTCTTTCTGCCTAACGTTTTGTTTATGATATTTGCCTGGCGTCACCAGGCGCATACACCAGCGAAAGGCCGGGTGGCCTGGACATTCGCATTTGGCGAAGCTTTCAAAGT > W3110S.gb/3714357‑3714490 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |