| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,783,965 | C→T | 9.2% | G13D (GGC→GAC) | yidI ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,783,965 | 0 | C | T | 9.2% | 237.9 / 10.2 | 76 | G13D (GGC→GAC) | yidI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (37/32); new base T (4/3); total (41/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAAAAGCAT > W3110S.gb/3783907‑3784033 | aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACt > 1:2011648/1‑69 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACt > 1:3478166/1‑69 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACt > 1:3001710/1‑69 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATg < 1:2879744/65‑1 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATg < 1:2230399/65‑1 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACt > 1:2311487/1‑69 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACt > 1:2293066/1‑69 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACt > 1:1841737/1‑69 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACt > 1:2341720/1‑69 (MQ=255)aGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACt > 1:3178181/1‑69 (MQ=255) gAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGatttt > 1:1552717/1‑69 (MQ=255) aTGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGccc > 1:948446/1‑53 (MQ=255) aaaaGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGccc > 1:92261/1‑50 (MQ=255) aaaGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTg > 1:1628197/1‑69 (MQ=255) aaaGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTg > 1:3498456/1‑69 (MQ=255) aaGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTg < 1:737551/68‑1 (MQ=255) aaGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGTCCAATGAACTGATTTTATTTTg < 1:3180220/68‑1 (MQ=255) gTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGc > 1:3615186/1‑69 (MQ=255) tGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCt < 1:1123638/69‑1 (MQ=255) gATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt > 1:2979694/1‑69 (MQ=255) gATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:945067/69‑1 (MQ=255) tAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGa > 1:2250794/1‑69 (MQ=255) aaTCCCCATCATTAAAGCGATGGCACCGAACAGCATGccc < 1:2619725/40‑1 (MQ=255) tCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCt < 1:1995475/62‑1 (MQ=255) ccccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATAcc < 1:760733/69‑1 (MQ=255) cccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCt < 1:3105886/60‑1 (MQ=255) cccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCt < 1:1287100/60‑1 (MQ=255) cccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCt < 1:2882076/60‑1 (MQ=255) cccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCt < 1:3239927/60‑1 (MQ=255) cccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATAccc < 1:2232037/69‑1 (MQ=255) cccATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATAccc < 1:2829812/69‑1 (MQ=255) tcatTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt > 1:2239441/1‑69 (MQ=255) tcatTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt > 1:3569281/1‑69 (MQ=255) tcatTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt > 1:1852061/1‑69 (MQ=255) tcatAAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATc > 1:3657483/1‑68 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:972595/56‑1 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt < 1:2632697/67‑1 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt < 1:143456/67‑1 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt > 1:373790/1‑67 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt < 1:1603831/67‑1 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGa > 1:881356/1‑69 (MQ=255) atTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGa > 1:316494/1‑69 (MQ=255) tAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGAcc > 1:553431/1‑69 (MQ=255) tAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGAcc > 1:2138343/1‑69 (MQ=255) aaaGCGATGGCACCGAACAGCATGCCCAATGAACTGatttta > 1:121694/1‑42 (MQ=255) aaaGCGATGGCACCGAACAGCATGCCCAATGAACTGatttta > 1:2975270/1‑42 (MQ=255) aaaGCGATGGCACCGAACAGCATGCCCAATGAACTGatttta > 1:3462947/1‑42 (MQ=255) aaaGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:1144089/53‑1 (MQ=255) aaaGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCt > 1:1181008/1‑64 (MQ=255) aaaGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTg < 1:728637/65‑1 (MQ=255) aaaGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTg > 1:3426301/1‑65 (MQ=255) ggCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAg > 1:3151172/1‑69 (MQ=255) ggCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAg > 1:2730537/1‑69 (MQ=255) ggCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAg > 1:3212836/1‑69 (MQ=255) ggCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAg > 1:2371312/1‑69 (MQ=255) aCCGAACAGCATGTCCAATGAACTGATTTTATTTTg < 1:1535760/36‑1 (MQ=255) aCCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCg < 1:2304548/68‑1 (MQ=255) aCCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCg < 1:3465123/68‑1 (MQ=255) aCCGAACAGCATGCCCAATGAACTGATTATATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCg < 1:3353999/68‑1 (MQ=255) aCCGAACAGCATGCCCAATGAACTGATTATATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCg < 1:3358338/68‑1 (MQ=255) ccGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCAt > 1:87181/1‑50 (MQ=255) ccGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCAt > 1:2017371/1‑50 (MQ=255) ccGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCg < 1:2582939/67‑1 (MQ=255) gAACAGTATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGAt > 1:477934/1‑68 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:2478120/39‑1 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:2638098/39‑1 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:223677/39‑1 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt > 1:41233/1‑39 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt > 1:3680749/1‑39 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt > 1:3149061/1‑39 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:3017908/39‑1 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGAt > 1:15978/1‑68 (MQ=255) gAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACATCCTTGTAGCGGAt > 1:190951/1‑68 (MQ=255) aaCAGCATGTCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATg > 1:3103418/1‑68 (MQ=255) aaCAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:1834435/38‑1 (MQ=255) aaCAGCATGCCCAATGAACTGATTTTATTTTGAGCTAt < 1:1849883/38‑1 (MQ=255) aaCAGCATGCCCAATGAACTGA‑TTTATTTTGAGCTAt < 1:630629/37‑1 (MQ=38) aTGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAAAAg < 1:856530/69‑1 (MQ=255) gCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTACCGGATGGCAAAAGCa < 1:3342196/69‑1 (MQ=255) cccAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAAAAGCat > 1:2101414/1‑69 (MQ=255) cccAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAAAAGCat < 1:2536834/69‑1 (MQ=255) cccAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAAAAGCat < 1:1993649/69‑1 (MQ=255) | AGGGCCGAATGAAAAGTGGATAATCCCCATCATTAAAGCGATGGCACCGAACAGCATGCCCAATGAACTGATTTTATTTTGAGCTATGATACCCATCTGACCTCCTTGTAGCGGATGGCAAAAGCAT > W3110S.gb/3783907‑3784033 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |