| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,815,914 | T→C | 82.6% | N702D (AAT→GAT) | spoT ← | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,815,914 | 0 | T | C | 82.6% | 110.6 / 14.9 | 46 | N702D (AAT→GAT) | spoT | bifunctional (p)ppGpp synthetase II and guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base T (6/2); new base C (16/22); total (22/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCAGATG > W3110S.gb/3815848‑3815980 | cAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAAtttc > 1:631748/1‑69 (MQ=255)cAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAAtctc > 1:3035523/1‑69 (MQ=255)cAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAAtctc > 1:3348038/1‑69 (MQ=255)cAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAAtctc > 1:2156618/1‑69 (MQ=255)cAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAAtctc > 1:2198013/1‑69 (MQ=255)cAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAAtctc > 1:2491131/1‑69 (MQ=255) ggCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCGTAAAACATTAAtttcgg > 1:291257/1‑69 (MQ=255) gccTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCggg < 1:1268273/69‑1 (MQ=255) gccTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCggg < 1:3563388/69‑1 (MQ=255) aGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTa < 1:837808/69‑1 (MQ=255) aGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTa < 1:1952955/69‑1 (MQ=255) aGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTa < 1:3171509/69‑1 (MQ=255) gCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTa < 1:561146/68‑1 (MQ=255) tCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg > 1:980313/1‑69 (MQ=255) tCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg > 1:2663784/1‑69 (MQ=255) tCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg > 1:3404082/1‑69 (MQ=255) tCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg > 1:431332/1‑69 (MQ=255) gCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCAt > 1:2133706/1‑69 (MQ=255) gCGTGCATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCAt > 1:2978902/1‑69 (MQ=255) cATAACGTGTTGGGTTCATAAAACATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCAcg > 1:209652/1‑68 (MQ=255) cATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACttt > 1:2091781/1‑49 (MQ=255) cATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCAcg > 1:537793/1‑68 (MQ=255) cATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCAcg > 1:3257379/1‑68 (MQ=255) cATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCAcg > 1:1080965/1‑68 (MQ=255) cATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCAcg > 1:1286970/1‑68 (MQ=255) cATAACGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTAAATCACGTCTGGCATCAcg > 1:3313651/1‑68 (MQ=255) aCGTGTTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGAt > 1:1714637/1‑69 (MQ=255) gtgtTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGAtt < 1:1056323/68‑1 (MQ=255) gtgtTGGGTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGAtt < 1:2732921/68‑1 (MQ=255) gggTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg < 1:748089/45‑1 (MQ=255) gggTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg < 1:2166795/45‑1 (MQ=255) gggTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg < 1:2460044/45‑1 (MQ=255) gggTTCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTgcg < 1:912048/68‑1 (MQ=255) ttCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCAt < 1:2936484/68‑1 (MQ=255) ttCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCAt < 1:718839/68‑1 (MQ=255) ttCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCAt < 1:2766642/68‑1 (MQ=255) tCATAAAACATTAATTTCGGTTTCGGGTGACTTTaa < 1:799064/36‑1 (MQ=255) tCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACg < 1:1319233/41‑1 (MQ=255) tCATAAAACATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGa > 1:683018/1‑69 (MQ=255) cATAAAACATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGat > 1:947142/1‑69 (MQ=255) aaaCATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGat > 1:2971227/1‑65 (MQ=255) aaaCATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTc > 1:3685568/1‑69 (MQ=255) aaaCATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTc > 1:2679624/1‑69 (MQ=255) aaaCATTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTc > 1:3328325/1‑69 (MQ=255) aCATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTc > 1:711844/1‑67 (MQ=255) aCATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTc > 1:3172288/1‑67 (MQ=255) aTTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCa < 1:3177787/69‑1 (MQ=255) aTTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCa < 1:3727924/69‑1 (MQ=255) aTTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCa < 1:886655/69‑1 (MQ=255) aTTAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCa < 1:3669946/69‑1 (MQ=255) ttAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCAg < 1:3387822/69‑1 (MQ=255) ttAATCTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCAg < 1:2464042/69‑1 (MQ=255) tAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCa < 1:2366809/67‑1 (MQ=255) ttcggtttcggGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCAGATg < 1:631178/67‑1 (MQ=255) | CAGGCTGCCGCCTGGCGAGCATTTCGCAGATGCGTGCATAACGTGTTGGGTTCATAAAACATTAATTTCGGTTTCGGGTGACTTTAATCACGTCTGGCATCACGCGGATTTTGCGCATGATATTCGCCAGATG > W3110S.gb/3815848‑3815980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |