| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,007,539 | G→A | 14.3% | pseudogene (367/393 nt) | yhiK → | ECK3474:JW5674:b3489; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,007,539 | 0 | G | A | 14.3% | 71.6 / 4.7 | 28 | pseudogene (367/393 nt) | yhiK | ECK3474:JW5674:b3489; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (11/13); new base A (2/2); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01 | |||||||||||
GTATTATGGGGCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTATGTCTTTTTAATCCTGC > W3110S.gb/4007474‑4007603 | gTATTATGGGGCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAagg > 1:3492676/1‑68 (MQ=255) atGGGGCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAgt > 1:3067472/1‑69 (MQ=255) atGGGGCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAgt > 1:3700502/1‑69 (MQ=255) gggCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCt > 1:2327769/1‑62 (MQ=255) gggCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAgttgt > 1:2979706/1‑69 (MQ=255) gggCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAgttg < 1:760277/68‑1 (MQ=255) tCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAAGGCTTAGTTGTTTTCAACTa < 1:3373342/69‑1 (MQ=255) tCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAAGGCTTAGTTGTTTTCAACTa < 1:3376719/69‑1 (MQ=255) aggaagCACGTGATAAAAATAATAAAATAATTTATTCCTCAAGGCTTAGTTGTTTTCAACTACATTaaa > 1:3107564/1‑69 (MQ=255) agCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTc < 1:1672196/69‑1 (MQ=255) agCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTc < 1:3101942/69‑1 (MQ=255) agCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTT‑AACTACATTAAAACTcc < 1:3147617/69‑1 (MQ=255) gCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTcc < 1:1605658/69‑1 (MQ=255) cGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATg < 1:877650/69‑1 (MQ=255) cGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATg < 1:1006456/69‑1 (MQ=255) aTAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATa > 1:1797345/1‑69 (MQ=255) aTAAAAATAATAAAATAATTTATTCCTCAAGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATa > 1:3178248/1‑69 (MQ=255) ataataaaataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACggtgg > 1:366390/1‑69 (MQ=255) ataataaaataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACggtgg < 1:3356414/69‑1 (MQ=255) ataaaataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTAt > 1:999338/1‑69 (MQ=255) ataaaataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTAt > 1:3020047/1‑69 (MQ=255) aaataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACt > 1:3128579/1‑48 (MQ=255) ataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACggtggt < 1:3423219/62‑1 (MQ=255) ataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACggtggt < 1:231332/62‑1 (MQ=255) ataatTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACggtggt < 1:1209672/62‑1 (MQ=255) tCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTATGTCTTTTTAATCCTGc < 1:652575/68‑1 (MQ=255) tCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTATGTCTTTTTAATCCTGc < 1:138982/68‑1 (MQ=255) cAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTATGTCTTTTTAAt > 1:2650972/1‑62 (MQ=255) cAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTATGTCTTTTTAAt > 1:1051378/1‑62 (MQ=255) | GTATTATGGGGCTAAATTCCCTGAAGGAAGCACGTGATAAAAATAATAAAATAATTTATTCCTCAGGGCTTAGTTGTTTTCAACTACATTAAAACTCCATGAATACGGTGGTATGTCTTTTTAATCCTGC > W3110S.gb/4007474‑4007603 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |