Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,031,980 T→C 46.7% N663D (AAC→GAC)  zntA ← zinc, cobalt and lead efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,031,9800TC46.7% ‑0.7 / 40.3 30N663D (AAC→GAC) zntAzinc, cobalt and lead efflux system
Reads supporting (aligned to +/- strand):  ref base T (5/11);  new base C (3/11);  total (8/22)
Fisher's exact test for biased strand distribution p-value = 6.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATCCC  >  W3110S.gb/4031915‑4032043
                                                                 |                                                               
cTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATg                                                              >  1:2605625/1‑69 (MQ=255)
cTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATg                                                              >  1:2060/1‑69 (MQ=255)
  ggCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATgg                                                             >  1:1243875/1‑68 (MQ=255)
     ggATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGtt                                                          <  1:2076225/68‑1 (MQ=255)
      gATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTaa                                                        <  1:47263/69‑1 (MQ=255)
      gATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTaa                                                        <  1:2313339/69‑1 (MQ=255)
      gATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTaa                                                        <  1:1536700/69‑1 (MQ=255)
          ttGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAAtgct                                                    >  1:3229415/1‑69 (MQ=255)
          ttGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAAtgct                                                    >  1:2242432/1‑69 (MQ=255)
                  aGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTcggc                                            <  1:3594035/69‑1 (MQ=255)
                  aGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTcggc                                            <  1:3515492/69‑1 (MQ=255)
                  aGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTcggc                                            <  1:2591312/69‑1 (MQ=255)
                  aGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTcggc                                            <  1:1267183/69‑1 (MQ=255)
                      gcgcGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGtt                                        >  1:1052871/1‑69 (MQ=255)
                      gcgcGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGtt                                        >  1:297803/1‑69 (MQ=255)
                               aGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGcc                               <  1:2041188/69‑1 (MQ=255)
                                     aTCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                          <  1:2630965/68‑1 (MQ=255)
                                     aTCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                          <  1:366731/68‑1 (MQ=255)
                                     aTCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                          <  1:208610/68‑1 (MQ=255)
                                     aTCATTTGCACCAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                          <  1:2010696/68‑1 (MQ=255)
                                     aTCATTTGCACCAGGCAGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTc                          <  1:1719584/68‑1 (MQ=255)
                                             cACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGc                  <  1:1680085/68‑1 (MQ=255)
                                             cACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGc                  <  1:2857709/68‑1 (MQ=255)
                                               ccAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTAc               <  1:3063163/69‑1 (MQ=255)
                                               ccAGGCCGCGCAGGTGGTCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTAc               <  1:3346297/69‑1 (MQ=255)
                                                      gcgcAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGc        <  1:369878/69‑1 (MQ=255)
                                                            gtggtTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc  <  1:1937086/69‑1 (MQ=255)
                                                              ggtCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc  <  1:1316924/67‑1 (MQ=255)
                                                              ggtCATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATccc  <  1:3300352/67‑1 (MQ=255)
                                                               gtTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCtgtg                      >  1:1462406/1‑46 (MQ=255)
                                                                 |                                                               
CTGGCGGATATTGGCGTGAGTGGCGCGTGCCAGTTCAATCATTTGCACCAGGCCGCGCAGGTGGTTATGGGTTAATGCTGCGTCGGCGGTTTCCAGCGCCACGTCTGTGCCGCTACCCATTGCAATCCC  >  W3110S.gb/4031915‑4032043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: