| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,066,786 | C→T | 66.7% | intergenic (+144/‑129) | asd → / → glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,066,786 | 0 | C | T | 66.7% | 56.0 / 45.1 | 51 | intergenic (+144/‑129) | asd/glgB | aspartate‑semialdehyde dehydrogenase, NAD(P)‑binding/1,4‑alpha‑glucan branching enzyme |
| Reads supporting (aligned to +/- strand): ref base C (11/6); new base T (21/13); total (32/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCAAAAA > W3110S.gb/4066726‑4066849 | gggAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTg < 1:1197606/69‑1 (MQ=255)gggAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTg < 1:815955/69‑1 (MQ=255)gggAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTg < 1:1827686/69‑1 (MQ=255)gggAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTg < 1:164239/69‑1 (MQ=255) gTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGt < 1:2624546/69‑1 (MQ=255) aaaTAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAg < 1:2938417/69‑1 (MQ=255) tAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGgac < 1:739917/69‑1 (MQ=255) agagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt > 1:3688771/1‑53 (MQ=255) agagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCtt > 1:95481/1‑53 (MQ=255) agagCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCt > 1:3253520/1‑52 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAgg > 1:2119584/1‑58 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagag > 1:161936/1‑69 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagag > 1:1485086/1‑69 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagag > 1:2083543/1‑69 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTAACTGAAGTGAgg > 1:3548340/1‑58 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCAAAGTCTTACCTg > 1:892856/1‑50 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAG‑ATTCACAGCCTTACCTGAAGTGAGGACGACGCagag > 1:1545108/1‑68 (MQ=255) ggCTATTCTTTAAGGGTGGCTGAATACATGAG‑ATTCACAGCCTTACCTGAAGTGAGGACGACGCagag > 1:2744748/1‑68 (MQ=255) tATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:474670/68‑1 (MQ=255) tATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:1150779/68‑1 (MQ=255) tATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCagagag < 1:1929793/68‑1 (MQ=255) aTTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGa > 1:2336785/1‑69 (MQ=255) tAAGGGTGGCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGca > 1:2619873/1‑67 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtg < 1:2839147/69‑1 (MQ=255) gTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtg < 1:3317941/69‑1 (MQ=255) gCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgctg > 1:2504813/1‑69 (MQ=255) gCTGAATACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGtgctg > 1:2762502/1‑69 (MQ=255) aTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg > 1:967182/1‑69 (MQ=255) aTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg > 1:1439812/1‑69 (MQ=255) aTACATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg > 1:1585697/1‑69 (MQ=255) tACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg > 1:115318/1‑68 (MQ=255) tACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCg > 1:844601/1‑68 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGacgacg > 1:3037346/1‑38 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTc > 1:1845399/1‑69 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTc > 1:325959/1‑69 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTc > 1:3373570/1‑69 (MQ=255) cATGAGTATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTc > 1:71085/1‑69 (MQ=255) aGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAgg > 1:1730094/1‑68 (MQ=255) aGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAgg > 1:948786/1‑68 (MQ=255) tATTCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCa < 1:3042673/69‑1 (MQ=255) ttCACAGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaa > 1:1639430/1‑68 (MQ=255) cacaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaa < 1:2288347/69‑1 (MQ=255) cacaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaa > 1:1427551/1‑69 (MQ=255) cacaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaa > 1:478300/1‑69 (MQ=255) cacaGTCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaa < 1:1923747/69‑1 (MQ=255) cacaGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATg < 1:568502/39‑1 (MQ=255) cacaGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATg < 1:3672454/39‑1 (MQ=255) cacaGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCTTTCAGGTCaaaaa < 1:417861/69‑1 (MQ=255) cacaGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaa > 1:2226293/1‑69 (MQ=255) cacaGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaa > 1:1012271/1‑69 (MQ=255) cacaGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCaaaaa < 1:349472/69‑1 (MQ=255) | GGGAGTTAAATAGAGCATTGGCTATTCTTTAAGGGTGGCTGAATACATGAGTATTCACAGCCTTACCTGAAGTGAGGACGACGCAGAGAGGATGCACAGAGTGCTGCGCCGTTCAGGTCAAAAA > W3110S.gb/4066726‑4066849 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |