| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,103,846 | T→C | 30.3% | Y5H (TAC→CAC) | ompR → | DNA‑binding response regulator in two‑component regulatory system with EnvZ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,103,846 | 0 | T | C | 30.3% | 103.1 / 64.6 | 76 | Y5H (TAC→CAC) | ompR | DNA‑binding response regulator in two‑component regulatory system with EnvZ |
| Reads supporting (aligned to +/- strand): ref base T (31/22); new base C (15/8); total (46/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
AGCTTTTTTAAGAATACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCGAACA > W3110S.gb/4103778‑4103913 | aGCTTTTTTAAGAATACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACt < 1:163228/69‑1 (MQ=255) ttAAGAATACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGAt > 1:2676024/1‑69 (MQ=255) ttAAGAATACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGAt > 1:3165773/1‑69 (MQ=255) tAAGAATACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGAt < 1:617886/68‑1 (MQ=255) aaTACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGa < 1:2386615/63‑1 (MQ=255) aaTACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtg < 1:846081/68‑1 (MQ=255) tACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCtggtg > 1:710344/1‑69 (MQ=255) tACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCtggtg > 1:2317006/1‑69 (MQ=255) tACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtg > 1:271940/1‑69 (MQ=255) tACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtg > 1:598699/1‑69 (MQ=255) tACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtg > 1:403129/1‑69 (MQ=255) tACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggt > 1:1054750/1‑68 (MQ=255) cacGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTc > 1:3646640/1‑62 (MQ=255) cacGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCtggtg > 1:414461/1‑67 (MQ=255) cacGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCtggtg > 1:3550139/1‑67 (MQ=255) cacGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCtggtgg > 1:2345046/1‑68 (MQ=255) cGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTc < 1:192652/68‑1 (MQ=255) cGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTc < 1:1586654/68‑1 (MQ=255) cGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTc < 1:619445/68‑1 (MQ=255) cGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTc < 1:2645028/68‑1 (MQ=255) cGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTc < 1:3097517/68‑1 (MQ=255) cGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTc < 1:603595/68‑1 (MQ=255) gCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTc < 1:2534332/67‑1 (MQ=255) cAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgac > 1:1764235/1‑68 (MQ=255) aaaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgac < 1:3691467/67‑1 (MQ=255) aaaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgac < 1:3036390/67‑1 (MQ=255) aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga < 1:438446/68‑1 (MQ=255) aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga < 1:1572644/68‑1 (MQ=255) aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATgacga < 1:2908401/68‑1 (MQ=255) aaTTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATgacga < 1:103249/68‑1 (MQ=255) ttgttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCtgg > 1:287606/1‑53 (MQ=255) ttgttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga > 1:1061953/1‑66 (MQ=255) ttgttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga > 1:663922/1‑66 (MQ=255) ttgttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga > 1:1100117/1‑66 (MQ=255) ttgttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga > 1:2552281/1‑66 (MQ=255) ttgttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATgacga > 1:1709023/1‑66 (MQ=255) gttgCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATgc > 1:735194/1‑69 (MQ=255) cGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGa < 1:740758/41‑1 (MQ=255) aCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCg > 1:3092997/1‑50 (MQ=255) aCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCg > 1:630631/1‑50 (MQ=255) aCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCg > 1:1349399/1‑50 (MQ=255) aCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCg > 1:2963555/1‑50 (MQ=255) aCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCg > 1:2924411/1‑50 (MQ=255) aCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCtgcg < 1:3690433/69‑1 (MQ=255) aCCTTTGGGAGTACAAACAATGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCtgcg < 1:912826/69‑1 (MQ=255) tttGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCtgcgtgc > 1:2583578/1‑69 (MQ=255) tttGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCtgcgtgc > 1:2466581/1‑69 (MQ=255) acaaacaaTGCAAGAGAACTACAAGATTCTGGTGGTCg < 1:2702991/38‑1 (MQ=255) acaaacaaTGCAAGAGAACTACAAGATTCTGGTGGTCg < 1:2020308/38‑1 (MQ=255) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTc > 1:1196887/1‑36 (MQ=255) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTc > 1:2414050/1‑36 (MQ=255) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTc > 1:2376274/1‑36 (MQ=255) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATgacgac < 1:12075/45‑1 (MQ=255) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATgacgac < 1:1248125/45‑1 (MQ=255) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATgacgac < 1:1559358/45‑1 (MQ=255) caaacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGAGGACGACATGCGCCTGCGTGCGCTGCTGGaa > 1:2325689/1‑69 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa > 1:1202672/1‑69 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa > 1:3324104/1‑69 (MQ=255) caaacaaTGCAAGAGAACCACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGaa > 1:857086/1‑69 (MQ=255) aacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCgct > 1:2915868/1‑60 (MQ=255) aacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCgct > 1:2126381/1‑60 (MQ=255) aacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCgct > 1:3151839/1‑60 (MQ=255) aacaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAAc > 1:3465860/1‑68 (MQ=255) acaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTgcgc > 1:3709745/1‑58 (MQ=255) acaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTgcgc > 1:1180300/1‑58 (MQ=255) acaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGt < 1:401592/69‑1 (MQ=255) acaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGt > 1:2455705/1‑69 (MQ=255) acaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGt < 1:3390938/69‑1 (MQ=255) acaaTGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGt > 1:3336717/1‑69 (MQ=255) cAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAtctc > 1:1495251/1‑69 (MQ=255) cAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAtctc < 1:600070/69‑1 (MQ=255) cAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTAtctc < 1:120799/69‑1 (MQ=255) gagaACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTgg < 1:1236527/55‑1 (MQ=255) gagaACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTgg < 1:1232007/55‑1 (MQ=255) gaACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCg > 1:2709279/1‑68 (MQ=255) gaACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCg > 1:3491993/1‑68 (MQ=255) cTACAAGAATCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCGAACa > 1:1075370/1‑69 (MQ=255) tACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCGAACa < 1:1339800/68‑1 (MQ=255) | AGCTTTTTTAAGAATACACGCTTACAAATTGTTGCGAACCTTTGGGAGTACAAACAATGCAAGAGAACTACAAGATTCTGGTGGTCGATGACGACATGCGCCTGCGTGCGCTGCTGGAACGTTATCTCACCGAACA > W3110S.gb/4103778‑4103913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |