Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 344,381 | G→A | 72.4% | intergenic (+166/‑247) | yahL → / → yahM | hypothetical protein/hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 344,381 | 0 | G | A | 72.4% | 88.8 / 41.9 | 58 | intergenic (+166/‑247) | yahL/yahM | hypothetical protein/hypothetical protein |
Reads supporting (aligned to +/- strand): ref base G (8/8); new base A (22/20); total (30/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGAC > W3110S.gb/344314‑344446 | ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGt > 1:263721/1‑69 (MQ=255) ccATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGt > 1:178921/1‑69 (MQ=255) tctcTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGAttt < 1:587919/68‑1 (MQ=255) tctcTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGAttt > 1:3575790/1‑68 (MQ=255) tctcTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGAttt > 1:3539795/1‑68 (MQ=255) ctctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGttt > 1:225516/1‑67 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGc > 1:820921/1‑69 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGc > 1:1947419/1‑69 (MQ=255) tctTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGc > 1:1676675/1‑69 (MQ=255) ttttCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGCAGcc > 1:1826268/1‑69 (MQ=255) ttCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGc < 1:2988766/66‑1 (MQ=255) ttgttgTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaa > 1:431810/1‑68 (MQ=255) ttgttgTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaa > 1:1051135/1‑68 (MQ=255) ttgttgTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaa > 1:2845936/1‑68 (MQ=255) ttgtGATCGGCGACACTTCGCTTAAAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATaaaa < 1:2220578/68‑1 (MQ=255) gtgATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATg < 1:1720384/69‑1 (MQ=255) gtgATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATg < 1:1688298/69‑1 (MQ=255) gtgATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATg < 1:1969782/69‑1 (MQ=255) gtgATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATg < 1:1166779/69‑1 (MQ=255) gtgATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATg < 1:202530/69‑1 (MQ=255) cGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:306709/68‑1 (MQ=255) cGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGAtct > 1:2625854/1‑68 (MQ=255) cGGCGAAACTTCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:1776075/68‑1 (MQ=255) ggCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:1332750/67‑1 (MQ=255) ggCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCg > 1:2959875/1‑49 (MQ=255) ggCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCg > 1:18165/1‑49 (MQ=255) ggCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCg > 1:752479/1‑49 (MQ=255) acacTTCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCa < 1:2192334/69‑1 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:1981529/69‑1 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGCACCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:820227/69‑1 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCa > 1:2143689/1‑36 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCa > 1:2891085/1‑36 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCa > 1:1893463/1‑36 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCa < 1:2199425/36‑1 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATg < 1:122464/68‑1 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATg < 1:882794/68‑1 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATg < 1:1770929/68‑1 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt > 1:675624/1‑69 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt > 1:2141245/1‑69 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt > 1:981506/1‑69 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt > 1:2935419/1‑69 (MQ=255) tCGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGt < 1:2690112/69‑1 (MQ=255) cGCTT‑AAAAAAGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGtt > 1:2764219/1‑69 (MQ=255) aaaaaaGCACCAGTAGTGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg > 1:781875/1‑69 (MQ=255) aaaaGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:95379/67‑1 (MQ=255) aaaGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg > 1:3549780/1‑66 (MQ=255) gCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAAtgtg < 1:3402237/69‑1 (MQ=255) gCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAAtgtg < 1:2259747/69‑1 (MQ=255) cACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAAtg > 1:1887495/1‑66 (MQ=255) tagtGGTTTCGCAGCCATGCGGTGTATAAAAAATGAtct < 1:88739/39‑1 (MQ=255) tagtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:1240590/56‑1 (MQ=255) tagtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:2678680/56‑1 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:3523810/54‑1 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:1289276/54‑1 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGAc > 1:2999137/1‑69 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGAc > 1:1350734/1‑69 (MQ=255) gtGATTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGAc > 1:3024556/1‑69 (MQ=255) gtGATTTAGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTtgtg < 1:2047237/54‑1 (MQ=255) | CCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGCGACACTTCGCTT‑AAAAAAGCACCAGTAGTGGTTTCGCAGCCATGCGGTGTATAAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGAC > W3110S.gb/344314‑344446 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |