| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,124,493 | T→C | 47.6% | intergenic (+95/‑12) | damX → / → dam | hypothetical protein/DNA adenine methylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,124,493 | 0 | T | C | 47.6% | 2.2 / 56.0 | 42 | intergenic (+95/‑12) | damX/dam | hypothetical protein/DNA adenine methylase |
| Reads supporting (aligned to +/- strand): ref base T (8/14); new base C (3/17); total (11/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGC > W3110S.gb/4124429‑4124556 | tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAttagt < 1:3003051/68‑1 (MQ=255)tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAttagt < 1:2283000/68‑1 (MQ=255)tGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcagt < 1:683055/68‑1 (MQ=255) gCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAttagt < 1:2673190/67‑1 (MQ=255) tccTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGTAGAAGGTGTAAtcagt < 1:840385/57‑1 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCTGAGAAGGTGTAAtcagt < 1:1728201/58‑1 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcagt < 1:967978/58‑1 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcagt < 1:3719652/58‑1 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcagt > 1:124859/1‑58 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcagt < 1:320461/58‑1 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcagt < 1:2257564/58‑1 (MQ=255) gCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCa > 1:3069635/1‑67 (MQ=255) gcgcTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGaaaa < 1:843572/69‑1 (MQ=255) gATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAAtcagt < 1:2568513/45‑1 (MQ=255) tGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCt < 1:811527/69‑1 (MQ=255) tGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCt < 1:2327480/69‑1 (MQ=255) cGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATTGCGCttt < 1:2626417/65‑1 (MQ=255) cGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCttt < 1:908727/65‑1 (MQ=255) cGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCttt < 1:2775663/65‑1 (MQ=255) tgAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:685203/67‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGACGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGa < 1:495391/69‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:2025143/68‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:87298/68‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:623568/68‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:3635947/68‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:3405984/68‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:2644966/68‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCTCTTTTTTGa < 1:1630233/69‑1 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCttttt > 1:399727/1‑66 (MQ=255) ggAGCTTTCTCCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:3302632/68‑1 (MQ=255) ttCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCt > 1:950212/1‑56 (MQ=255) tctcCACAGCCGGAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGc > 1:3259147/1‑69 (MQ=255) cacaGCCGGAGAAGGTGTAATCAGTTAGTCAGCAt < 1:3102101/35‑1 (MQ=255) cacaGCCGGAGAAGGTGTAATCAGTTAGTCAGCAt < 1:250196/35‑1 (MQ=255) caGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGaaaa < 1:1642623/41‑1 (MQ=255) caGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGaaaa > 1:2204652/1‑41 (MQ=255) aGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:833694/54‑1 (MQ=255) aGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:852894/54‑1 (MQ=255) aGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTg < 1:442717/54‑1 (MQ=255) aGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGa > 1:267253/1‑55 (MQ=255) aGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGa > 1:1263537/1‑55 (MQ=255) cGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAg < 1:2243290/69‑1 (MQ=255) ggAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAg < 1:1170551/68‑1 (MQ=255) ggAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGt < 1:2619111/69‑1 (MQ=255) ggAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGt < 1:1051764/69‑1 (MQ=255) ggAGAAGGTGTAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGt < 1:876234/69‑1 (MQ=255) aaGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGa > 1:1595168/1‑47 (MQ=255) aaGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGa > 1:200191/1‑47 (MQ=255) tgtAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTg < 1:3596797/69‑1 (MQ=255) gtAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGc > 1:1196325/1‑69 (MQ=255) gtAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGc > 1:1422332/1‑69 (MQ=255) gtAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGaa < 1:891304/43‑1 (MQ=255) gtAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGaa < 1:1069115/43‑1 (MQ=255) gtAATCAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGaa < 1:1148459/43‑1 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGc > 1:397650/1‑45 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGc > 1:3530330/1‑45 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGc > 1:3433964/1‑45 (MQ=255) ttagttagTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGc > 1:246412/1‑45 (MQ=255) | TGCGGGAGTTGCCTGAAGCGCTGGATGCTGTCGGAGCTTTCTCCACAGCCGGAGAAGGTGTAATTAGTTAGTCAGCATGAAGAAAAATCGCGCTTTTTTGAAGTGGGCAGGGGGCAAGTATCCCCTGC > W3110S.gb/4124429‑4124556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |