| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,154,666 | A→G | 83.3% | I23V (ATT→GTT) | yhfA → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,154,666 | 0 | A | G | 83.3% | 102.6 / 12.9 | 42 | I23V (ATT→GTT) | yhfA | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (19/16); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
GCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTT > W3110S.gb/4154602‑4154721 | gCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:2138947/69‑1 (MQ=255) cAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTa > 1:2715604/1‑69 (MQ=255) aGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:127804/1‑68 (MQ=255) aGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:3348964/1‑68 (MQ=255) aGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa < 1:2781535/68‑1 (MQ=255) gcgcGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:2636865/69‑1 (MQ=255) cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:1310238/1‑66 (MQ=255) cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:182721/1‑66 (MQ=255) cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:1837427/1‑66 (MQ=255) cgcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTaa > 1:1405288/1‑66 (MQ=255) gcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATg < 1:1329758/67‑1 (MQ=255) gcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATg < 1:2557150/67‑1 (MQ=255) gcgAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATg > 1:3659977/1‑67 (MQ=255) gTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt > 1:742389/1‑59 (MQ=255) gTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:209926/59‑1 (MQ=255) gTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:373988/59‑1 (MQ=255) gTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTc < 1:2854387/69‑1 (MQ=255) gggTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCa > 1:434787/1‑68 (MQ=255) aGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATg < 1:1763118/49‑1 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGc > 1:90579/1‑68 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGc > 1:2725654/1‑68 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGc > 1:3016160/1‑68 (MQ=255) ttAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCa < 1:202953/69‑1 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATaaa > 1:2020778/1‑64 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATaa > 1:3207737/1‑63 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATaa > 1:3453963/1‑63 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:3297524/1‑65 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:912721/1‑65 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:1748509/1‑65 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg < 1:3296541/65‑1 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAg > 1:2025038/1‑65 (MQ=255) aaCTTTTCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCa < 1:1015893/67‑1 (MQ=255) tttCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:875998/35‑1 (MQ=255) tttCTGGGCGAATCCGCCTCTGGTCATCAGGtttt < 1:1851456/35‑1 (MQ=255) tttCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGAt > 1:451554/1‑57 (MQ=255) tttCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATg < 1:2248643/39‑1 (MQ=255) ttCTGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGt > 1:2495110/1‑68 (MQ=255) cTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATTGACGGCAACTCAGGCGATAAAGCACCAAGTcc < 1:2842155/68‑1 (MQ=255) tGGGCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCg < 1:2919243/68‑1 (MQ=255) gggCGAATCCGCCTCTGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCg < 1:956458/67‑1 (MQ=255) ctctGGTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGtt < 1:2116210/69‑1 (MQ=255) gTCATCAGGTTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGgtg < 1:1459670/62‑1 (MQ=255) | GCAAGCGCGAGTGAAGTGGGTCGAAGGGTTAACTTTTCTGGGCGAATCCGCCTCTGGTCATCAGATTTTAATGGACGGCAACTCAGGCGATAAAGCACCAAGTCCGATGGAAATGGTGTT > W3110S.gb/4154602‑4154721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |