| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,182,652 | C→T | 29.4% | V464M (GTG→ATG) | gspD ← | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,182,652 | 0 | C | T | 29.4% | 48.8 / 23.1 | 34 | V464M (GTG→ATG) | gspD | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (10/14); new base T (7/3); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAGGAT > W3110S.gb/4182585‑4182718 | gTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGACGTGGTCTGTGACCCGGATAGCACCGGcaca < 1:983173/69‑1 (MQ=255)gTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGcaca < 1:1190201/69‑1 (MQ=255)gTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGcaca < 1:2247013/69‑1 (MQ=255)gTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGcaca < 1:1503949/69‑1 (MQ=255) ttttGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACAt < 1:2323878/69‑1 (MQ=255) ttttGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACAt < 1:3087512/69‑1 (MQ=255) ttGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATcc > 1:865821/1‑69 (MQ=255) cGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCt > 1:3294526/1‑60 (MQ=255) cGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTg > 1:3298990/1‑61 (MQ=255) aTTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATTGCACCGGCACAt < 1:3166131/53‑1 (MQ=255) aTTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACAt < 1:1914647/53‑1 (MQ=255) aTTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACAt < 1:228880/53‑1 (MQ=255) aTTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGa > 1:1752814/1‑68 (MQ=255) aTTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGa > 1:643393/1‑68 (MQ=255) ttAAAGACGTTATCCCATGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAg > 1:1636102/1‑69 (MQ=255) tATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGtt > 1:2797678/1‑69 (MQ=255) tATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGtt > 1:3627717/1‑69 (MQ=255) ccccTGAAGTGGTCTGTGACCCGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTttat > 1:1104272/1‑69 (MQ=255) gAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAg > 1:1038329/1‑59 (MQ=255) gAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAg > 1:2085998/1‑59 (MQ=255) gTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTttatta > 1:2679745/1‑60 (MQ=255) gTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGtt > 1:956112/1‑69 (MQ=255) gTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGtt > 1:1957700/1‑69 (MQ=255) gTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGtt > 1:2922756/1‑69 (MQ=255) gTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGtt > 1:1376678/1‑69 (MQ=255) cTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTAc < 1:1276471/69‑1 (MQ=255) gtgACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGttt < 1:3231262/52‑1 (MQ=255) gtgACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGttt < 1:1268698/52‑1 (MQ=255) gtgACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGttt < 1:3289815/52‑1 (MQ=255) gtgACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGttt < 1:2954850/52‑1 (MQ=255) ccGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCt < 1:1548381/68‑1 (MQ=255) ccGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCt < 1:3369710/68‑1 (MQ=255) ccGGATAGCACCGGCATATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCt < 1:1863583/68‑1 (MQ=255) ggATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTgg < 1:1282243/69‑1 (MQ=255) ggATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTgg < 1:3688606/69‑1 (MQ=255) gATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTg < 1:3183884/67‑1 (MQ=255) ccGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGc > 1:2644713/1‑67 (MQ=255) cGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAg < 1:1179563/69‑1 (MQ=255) acaTCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAGGAt > 1:2791363/1‑68 (MQ=255) | GTTTTGCGTTCGACGGTATTAAAGACGTTATCCCCTGAAGTGGTCTGTGACCCGGATAGCACCGGCACATCCTGCCCCACGTTGAAGGACGCGAGTTTATTATCCAGCGTTACGATGCTTGGGGTGGCGAGGAT > W3110S.gb/4182585‑4182718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |