| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,184,035 | C→A | 26.9% | G3* (GGA→TGA) R269M (AGG→ATG) |
gspD ← gspC ← |
general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,184,035 | 0 | C | A | 26.9% | 119.0 / 26.5 | 52 | G3* (GGA→TGA) R269M (AGG→ATG) | gspD gspC | general secretory pathway component, cryptic general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base C (29/9); new base A (4/10); total (33/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.85e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
CTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGCCG > W3110S.gb/4183970‑4184103 | ctcAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCttt < 1:965411/69‑1 (MQ=255)ctcAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCttt < 1:3687648/69‑1 (MQ=255) cGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCAt < 1:2608385/67‑1 (MQ=255) cGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCAt < 1:1462070/67‑1 (MQ=255) cacaAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTcc > 1:453771/1‑68 (MQ=255) ggCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcattcat < 1:2830605/69‑1 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACg > 1:850683/1‑51 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACg > 1:2849266/1‑51 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACg > 1:1614154/1‑51 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACg > 1:3254874/1‑51 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCt > 1:1822434/1‑67 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCt > 1:3400690/1‑67 (MQ=255) agtagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCt > 1:829166/1‑67 (MQ=255) tagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTa < 1:1297727/68‑1 (MQ=255) tagtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTa < 1:847745/68‑1 (MQ=255) agtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAg < 1:3581296/69‑1 (MQ=255) agtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAg < 1:2214830/69‑1 (MQ=255) agtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAg < 1:1290904/69‑1 (MQ=255) agtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGAAGCAGTCCATTCATTCCTGTAAg < 1:919480/69‑1 (MQ=255) gtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGt > 1:3386099/1‑69 (MQ=255) gtGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGt > 1:1159293/1‑69 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTcc > 1:1969366/1‑69 (MQ=255) ctgcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcc > 1:2829416/1‑69 (MQ=255) gcCAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTcccc < 1:3641956/69‑1 (MQ=255) ccAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTc > 1:401013/1‑65 (MQ=255) ccAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTCCTGTAAGTTc > 1:2173883/1‑65 (MQ=255) ccAGCAAGCAGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCCATTCATTACTGTAAGTTc > 1:2405577/1‑65 (MQ=255) agcaagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcat < 1:1391815/47‑1 (MQ=255) agcaagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcat < 1:2488586/47‑1 (MQ=255) agcaagcaGCAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcat < 1:1733136/47‑1 (MQ=255) aagcaGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCcatt > 1:3234853/1‑45 (MQ=255) aagcaGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCcatt > 1:47489/1‑45 (MQ=255) aagcaGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCcat > 1:2929182/1‑44 (MQ=255) aagcaGCAGGTGATTTTATTGAGACCTTTCATGACGCAGTCcatt > 1:699369/1‑45 (MQ=255) agcagcAGGTGATTTTATTGAGTCATTTCATGACGCAGTCcatt < 1:162891/44‑1 (MQ=255) ggTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAttt > 1:2660146/1‑68 (MQ=255) tGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTa > 1:2220109/1‑67 (MQ=255) gATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCa < 1:2076236/69‑1 (MQ=255) aTTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCaa > 1:3225805/1‑69 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCattcattc > 1:3567849/1‑35 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAATCAtt > 1:1235275/1‑60 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt > 1:3550336/1‑60 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt > 1:1704599/1‑60 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt > 1:3248867/1‑60 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt < 1:30319/60‑1 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt > 1:2913415/1‑60 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACAtt < 1:1547812/60‑1 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCg > 1:3549220/1‑69 (MQ=255) ttATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCg > 1:2287039/1‑69 (MQ=255) tATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgg > 1:3709874/1‑69 (MQ=255) tATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgg > 1:894710/1‑69 (MQ=255) tATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCgg > 1:1146247/1‑69 (MQ=255) cTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGCCg > 1:935288/1‑69 (MQ=255) | CTCAGCGTGTCCTGCACAAGGCATGAGTAGTGCTGCCAGCAAGCAGCAGGTGATTTTATTGAGTCCTTTCATGACGCAGTCCATTCATTCCTGTAAGTTCCCCGACGGAAACATTTATCAAGCGGGGTACGCCG > W3110S.gb/4183970‑4184103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |