| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,246,866 | T→C | 62.5% | V81V (GTA→GTG) | malG ← | maltose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,246,866 | 0 | T | C | 62.5% | 26.0 / 30.0 | 32 | V81V (GTA→GTG) | malG | maltose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (3/9); new base C (7/13); total (10/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTGGAGAGCGCCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGT > W3110S.gb/4246798‑4246929 | gtggAGAGCGCCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGt < 1:3050145/69‑1 (MQ=255)gtggAGAGCGCCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGt < 1:1144848/69‑1 (MQ=255) gcgcCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGa < 1:1663174/69‑1 (MQ=255) acaATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGc < 1:2797258/69‑1 (MQ=255) caATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCg > 1:1916305/1‑69 (MQ=255) gCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTgg < 1:3466489/69‑1 (MQ=255) ccAATCGCGGAAATCACGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCAc > 1:2336486/1‑53 (MQ=255) aaTCGCGGAAATCCCGTCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:3460974/69‑1 (MQ=255) aaTCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:1459198/69‑1 (MQ=255) aaTCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:193830/69‑1 (MQ=255) aaTCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:3505877/69‑1 (MQ=255) aaTCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:2536630/69‑1 (MQ=255) aaTCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:1209004/69‑1 (MQ=255) aaTCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:2412659/69‑1 (MQ=255) tCGCGGAAATCCCGGCGTCCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGt < 1:3019666/67‑1 (MQ=255) gcgGAAATCCCGGCGACCTTTACCGAGTTCCAAAGCCACAGCAGCACCggg < 1:202993/51‑1 (MQ=39) ggAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCg < 1:3631422/69‑1 (MQ=255) aaTCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGAcc > 1:2609279/1‑69 (MQ=255) aaTCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGAcc > 1:2940842/1‑69 (MQ=255) aTCCCGGCGCCCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCgg > 1:2067757/1‑53 (MQ=39) cccGGCGACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGAc < 1:2224141/65‑1 (MQ=255) gACCTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGAc < 1:2945840/59‑1 (MQ=255) cTTTACCGAGTTCCACAGCCACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTc > 1:2120992/1‑69 (MQ=255) cTTTACCGAGTTCCACAGACACAGCAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTc > 1:2440800/1‑69 (MQ=255) ttACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGt < 1:2509798/46‑1 (MQ=255) ttACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGt < 1:321719/46‑1 (MQ=255) tACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGt > 1:1286479/1‑45 (MQ=255) acagccacagcAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:1073746/68‑1 (MQ=255) acagccacagcAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:635007/68‑1 (MQ=255) acagccacagcAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:1337060/68‑1 (MQ=255) acagccacagcAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:1069388/68‑1 (MQ=255) acagccacagcAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCt < 1:1289400/68‑1 (MQ=255) cacagcAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAAc > 1:2890668/1‑61 (MQ=255) cacagcAGCACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAAc > 1:1978619/1‑61 (MQ=255) agcagcACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGt < 1:2471240/69‑1 (MQ=255) gcagcaCCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGt > 1:727855/1‑68 (MQ=255) | GTGGAGAGCGCCACAATGCCAATCGCGGAAATCCCGGCGACCTTTACCGAGTTCCACAGCCACAGCAGTACCGGGAATGGCGGTGGCGTAATGCGACCATCAGCCTGTTCAACGCTAAAACCTAACGCCAGT > W3110S.gb/4246798‑4246929 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |