| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 381,851 | A→C | 57.8% | G88G (GGT→GGG) | yaiF ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 381,851 | 0 | A | C | 57.8% | 52.3 / 40.7 | 45 | G88G (GGT→GGG) | yaiF | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/12); new base C (11/15); total (18/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
AGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGT > W3110S.gb/381786‑381913 | aGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGccc < 1:1664958/68‑1 (MQ=255) tCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAAt < 1:1114483/69‑1 (MQ=255) aaTATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACgg < 1:2546276/68‑1 (MQ=255) gggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACgg < 1:2528409/61‑1 (MQ=255) gggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACgg < 1:719755/61‑1 (MQ=255) aaaTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa < 1:1188890/69‑1 (MQ=255) aaaTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACaaa < 1:3464454/69‑1 (MQ=255) aaTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCAt < 1:1297183/62‑1 (MQ=255) aaTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCAt < 1:1623520/62‑1 (MQ=255) aTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAc < 1:3152681/58‑1 (MQ=255) aTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAc < 1:2144648/58‑1 (MQ=255) aTCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCAc < 1:2727807/58‑1 (MQ=255) tCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACa > 1:1921326/1‑64 (MQ=255) tCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATAc > 1:3280129/1‑69 (MQ=255) ccAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATaa < 1:3133366/50‑1 (MQ=255) ccAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAATaa < 1:28877/50‑1 (MQ=255) tACCTATCAGGCAGTTTGCGCCAATCACTACCGGCCCCTGAAt < 1:2906754/43‑1 (MQ=255) gCAGTTTGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGAt < 1:2466641/69‑1 (MQ=255) ttGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaa > 1:726669/1‑69 (MQ=255) ttGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaa > 1:2262473/1‑69 (MQ=255) ttGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaaca > 1:1164230/1‑68 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa > 1:2150325/1‑45 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa < 1:3651870/45‑1 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa > 1:2873664/1‑45 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa < 1:2092138/45‑1 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa < 1:1365182/45‑1 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa < 1:1382826/45‑1 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa > 1:3035310/1‑45 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa > 1:3188342/1‑45 (MQ=255) ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACaaa < 1:2269334/45‑1 (MQ=255) tGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACa > 1:1300003/1‑42 (MQ=255) tGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACa < 1:3556008/42‑1 (MQ=255) tGCGCCAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACggg < 1:3137654/50‑1 (MQ=255) ccAATCACTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCa < 1:3089969/69‑1 (MQ=255) ccAATCACTACCGGACCCTGAATAACGTCACCATGACAAATACGTGTATTCGCGCCGATAACAACTTc < 1:2025822/68‑1 (MQ=255) ccAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTc < 1:3028777/68‑1 (MQ=255) aCTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCa > 1:3147983/1‑69 (MQ=255) aCTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCa > 1:2082669/1‑69 (MQ=255) aCTACCGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCa > 1:1352096/1‑69 (MQ=255) cTACCGGCCCCTGAATAACGGCACCATGACAAATACggg > 1:2097167/1‑39 (MQ=255) cTACCGGCCCCTGAATAACGGCACCATGACAAATACggg > 1:2126460/1‑39 (MQ=255) cTACCGGCCCCTGAATAACGGCACCATGACAAATACggg > 1:803094/1‑39 (MQ=255) cTACCGGCCCCTGAATAACGGCACCATGACAAATACgg > 1:3652081/1‑38 (MQ=255) aCCGGACACTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGt > 1:1716342/1‑68 (MQ=255) ccGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaa < 1:2598610/54‑1 (MQ=255) ccGGCCCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATaacaa < 1:48517/54‑1 (MQ=255) | AGATGTCTGGAAATATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGT > W3110S.gb/381786‑381913 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |