| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,390,267 | A→G | 12.1% | N65D (AAC→GAC) | yjeN → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,390,267 | 0 | A | G | 12.1% | 81.2 / 4.9 | 33 | N65D (AAC→GAC) | yjeN | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/9); new base G (2/2); total (22/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGG > W3110S.gb/4390200‑4390330 | tAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGaaa < 1:1382213/69‑1 (MQ=255) ggCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGc > 1:3462539/1‑68 (MQ=255) ggtggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGata > 1:326405/1‑68 (MQ=255) ggtggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGata > 1:2962071/1‑68 (MQ=255) gtggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatt > 1:3349014/1‑69 (MQ=255) gtggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatt < 1:2973012/69‑1 (MQ=255) gtggtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatt > 1:2471717/1‑69 (MQ=255) gtggGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtattt < 1:253486/67‑1 (MQ=255) aGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAGACCTGCCGGATATTTATTGCGct < 1:103969/69‑1 (MQ=255) aGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtattt > 1:1115706/1‑61 (MQ=255) aGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtattt > 1:3164606/1‑61 (MQ=255) aGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtattt > 1:2025338/1‑61 (MQ=255) aGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtattt > 1:1844247/1‑61 (MQ=255) gCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGctt < 1:3429854/69‑1 (MQ=255) gCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGctt < 1:2728298/69‑1 (MQ=255) cACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGAtatt > 1:965699/1‑58 (MQ=255) actaTTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGct < 1:1135845/63‑1 (MQ=255) aCCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttat > 1:2131876/1‑69 (MQ=255) aTTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttatctt > 1:68854/1‑69 (MQ=255) aaCACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttatcttat > 1:3104327/1‑68 (MQ=255) aaCACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttatcttat > 1:1642523/1‑68 (MQ=255) aaCACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttatcttat > 1:581941/1‑68 (MQ=255) aaCACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGctt < 1:2568215/61‑1 (MQ=255) aGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGt > 1:1176580/1‑69 (MQ=255) aGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGt > 1:3519633/1‑69 (MQ=255) tCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttat < 1:3128151/49‑1 (MQ=255) tCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGcttat > 1:2203224/1‑49 (MQ=255) tCGCGATGGTAAACGGTGGGGAAACCTGACGGATATTTATTGCGcttat > 1:693134/1‑49 (MQ=255) cgcgATGGTAAACGGTGGGGAGACCTGCCGGAtatt > 1:2624381/1‑36 (MQ=255) cgcgATGGTAAACGGTGGGGAGACCTGCCGGAtatt > 1:916877/1‑36 (MQ=255) cGGTGGGGAGACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTa < 1:1425929/69‑1 (MQ=255) cGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAAtt < 1:1880529/68‑1 (MQ=255) ggggAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTgg < 1:3216072/69‑1 (MQ=255) ggggAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTgg > 1:741730/1‑69 (MQ=255) | TAAAGTGGCGATGGTGGTGGGTAGCACCATTAACACCCTCAAAGATCGCGATGGTAAACGGTGGGGAAACCTGCCGGATATTTATTGCGCTTATCTTATCCGATGTCTGGTTTTTCGCGGGGAATTAGTGG > W3110S.gb/4390200‑4390330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |