| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,486,264 | C→T | 84.4% | P752P (CCG→CCA) | valS ← | valyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,486,264 | 0 | C | T | 84.4% | 74.0 / 8.1 | 32 | P752P (CCG→CCA) | valS | valyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (2/3); new base T (10/17); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
GTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGT > W3110S.gb/4486198‑4486327 | gtCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTgg > 1:633329/1‑69 (MQ=255) tCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa < 1:542315/69‑1 (MQ=255) tCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa < 1:328534/69‑1 (MQ=255) tCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa < 1:2409287/69‑1 (MQ=255) tCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa < 1:1871639/69‑1 (MQ=255) tCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa < 1:1922148/69‑1 (MQ=255) aGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATgc > 1:2386341/1‑67 (MQ=255) gATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGa > 1:953049/1‑68 (MQ=255) gCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGgcgc > 1:3468788/1‑67 (MQ=255) aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCgcagca < 1:2126962/69‑1 (MQ=255) aaaGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCgcagca < 1:724558/69‑1 (MQ=255) aGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGa < 1:1992052/69‑1 (MQ=255) aGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGa < 1:2157270/69‑1 (MQ=255) gTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa < 1:1206638/50‑1 (MQ=255) gTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGAc < 1:754377/69‑1 (MQ=255) gTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGAc > 1:1018608/1‑69 (MQ=255) aCTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCt > 1:3522728/1‑69 (MQ=255) aCTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGcgcg < 1:1055248/54‑1 (MQ=255) cTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGcgcg < 1:612972/53‑1 (MQ=255) ttCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTcc > 1:2362695/1‑69 (MQ=255) acGCTGCCAGATGGTTTCGGTGATGAACGGAATGATTGGa < 1:2096454/40‑1 (MQ=255) tGGTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCg < 1:3268021/69‑1 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAg < 1:2397534/54‑1 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAg > 1:1959303/1‑54 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAg < 1:387069/54‑1 (MQ=255) ggTTTCGGTGATGAACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAg > 1:723087/1‑54 (MQ=255) tgatgaACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAg < 1:1031368/69‑1 (MQ=255) tgatgaACGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAg < 1:80831/69‑1 (MQ=255) gatgaACGGAATGATCGGATGAGCGAGGCGCAGCAGAACTTCCAGTACAGTCACCAGCGTATGGCg < 1:2165662/66‑1 (MQ=255) cGGAATGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTAcc > 1:3711121/1‑66 (MQ=255) aTGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGt > 1:1785912/1‑69 (MQ=255) aTGATTGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGt > 1:2431911/1‑69 (MQ=255) | GTCGGCAGTGATACCGCAAAGTACTTTCACACGCTGCCAGATGGTTTCGGTGATGAACGGAATGATCGGATGCGCGAGGCGCAGCAGACCTTCCAGTACAGTCACCAGCGTATGGCGAGTACCGCGCAGT > W3110S.gb/4486198‑4486327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |