| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,491,067 | G→A | 66.7% | V56V (GTG→GTA) | yjgP → | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,491,067 | 0 | G | A | 66.7% | 48.2 / 44.1 | 48 | V56V (GTG→GTA) | yjgP | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (12/4); new base A (16/16); total (28/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01 | |||||||||||
CCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGACGCTGGG > W3110S.gb/4491001‑4491129 | ccTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGc > 1:1011607/1‑68 (MQ=255) cGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGa < 1:1175212/69‑1 (MQ=255) ggCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCgg > 1:3196280/1‑67 (MQ=255) ggCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGa > 1:840343/1‑68 (MQ=255) ggCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGaa > 1:2941811/1‑69 (MQ=255) ggTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCa > 1:607164/1‑69 (MQ=255) gTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAg > 1:3410086/1‑69 (MQ=255) ttGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAg < 1:2008947/68‑1 (MQ=255) tGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGa > 1:577553/1‑57 (MQ=255) tGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGa > 1:2427154/1‑57 (MQ=255) gACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCTCa < 1:1684264/65‑1 (MQ=255) gACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCa < 1:1881092/65‑1 (MQ=255) gACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCa < 1:325634/65‑1 (MQ=255) cGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCTTa < 1:2952563/68‑1 (MQ=255) cGATATTCCGGCGAATCTTGTGCTCTCCCTTCTCGGGTTGGTCGTACCGGAAATGGCGCAGCTTATCCt < 1:3481166/69‑1 (MQ=255) cGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCt < 1:3607162/69‑1 (MQ=255) atTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGcc > 1:1897896/1‑68 (MQ=255) ttCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGc < 1:1714583/66‑1 (MQ=255) ccGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGgcg > 1:2680552/1‑51 (MQ=255) ccGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTa > 1:188805/1‑58 (MQ=255) ccGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTa > 1:999437/1‑69 (MQ=255) ccGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTa > 1:3345005/1‑69 (MQ=255) cGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGcc < 1:2444246/69‑1 (MQ=255) gAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGcc < 1:947441/68‑1 (MQ=255) tCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGtt > 1:2183417/1‑69 (MQ=255) tGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTaa > 1:2124324/1‑57 (MQ=255) tGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAg > 1:275035/1‑58 (MQ=255) tGCTCTCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAg > 1:17819/1‑58 (MQ=255) ctctCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCggg > 1:16612/1‑69 (MQ=255) ctctCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCggg > 1:2345201/1‑69 (MQ=255) ctctCCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCggg > 1:2576912/1‑69 (MQ=255) ctcCCTTCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGgct > 1:2851311/1‑69 (MQ=255) ccTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCt > 1:922153/1‑36 (MQ=255) ccTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCt > 1:2623167/1‑36 (MQ=255) ccTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCt > 1:572253/1‑36 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCTTGCCATTAAGCCTGt < 1:473867/54‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGt < 1:2261231/54‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGt < 1:2113462/54‑1 (MQ=255) ttCTCGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGgctgct < 1:3558551/67‑1 (MQ=255) tctcGGGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCtgat < 1:3592060/69‑1 (MQ=255) ctcGGGTTGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCtgatg > 1:1325772/1‑69 (MQ=255) tcGTGTTGGGCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGt < 1:2455764/51‑1 (MQ=255) tGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCgg < 1:489850/52‑1 (MQ=255) tGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCgg < 1:779692/52‑1 (MQ=255) tGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCgg < 1:1388554/52‑1 (MQ=255) gggCGTACCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCtgat > 1:3385383/1‑60 (MQ=255) ggCGTGCCGGAAATGGCGCAGCTTATCCTGCCAtt > 1:843846/1‑35 (MQ=255) ggCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGACGCTggg > 1:3132126/1‑68 (MQ=255) | CCTCGGCGCAGCGGTTGACGGCGATATTCCGGCGAATCTGGTGCTCTCCCTTCTCGGGTTGGGCGTGCCGGAAATGGCGCAGCTTATCCTGCCATTAAGCCTGTTCCTCGGGCTGCTGATGACGCTGGG > W3110S.gb/4491001‑4491129 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |