| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,547,549 | T→C | 17.4% | intergenic (+234/‑248) | fimE → / → fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,547,549 | 0 | T | C | 17.4% | 121.1 / 19.5 | 53 | intergenic (+234/‑248) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin |
| Reads supporting (aligned to +/- strand): ref base T (30/13); new base C (4/5); total (35/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01 | |||||||||||
TTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCC > W3110S.gb/4547486‑4547616 | ttCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctc > 1:3591291/1‑68 (MQ=255)ttCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctc > 1:743764/1‑68 (MQ=255) tCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctct > 1:2516112/1‑68 (MQ=255) tCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTtctct > 1:605449/1‑68 (MQ=255) tCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCAtctct > 1:1898831/1‑68 (MQ=255) tCACCTCCGCTATATGTAAAGCTAACGTTTGTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaaa < 1:1399229/69‑1 (MQ=255) cACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaaa > 1:3211764/1‑68 (MQ=255) cACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaaa > 1:2897202/1‑68 (MQ=255) aCCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCaaa > 1:610554/1‑66 (MQ=255) ccGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAaccacc < 1:3502457/69‑1 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCa < 1:1710378/68‑1 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCa < 1:1594357/68‑1 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCa < 1:36955/68‑1 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCAAAAac < 1:1605248/57‑1 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCAAAAac < 1:1891034/57‑1 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCAAAAac < 1:3423674/57‑1 (MQ=255) tGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCAAAAac < 1:2892447/57‑1 (MQ=255) tAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAat > 1:177905/1‑68 (MQ=255) tAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAat > 1:891321/1‑68 (MQ=255) aCGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAAtata < 1:1213624/63‑1 (MQ=255) aCGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAAtata < 1:2847316/63‑1 (MQ=255) aCGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAAtata < 1:238198/63‑1 (MQ=255) aCGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACAt > 1:2010435/1‑68 (MQ=255) gTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaaa > 1:2388821/1‑42 (MQ=255) gTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaaa > 1:3098749/1‑42 (MQ=255) gTTTCTGTGGCTCGACGCATCTTCCTCATTCCTCTCTCCaaa > 1:1800509/1‑42 (MQ=255) tttCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCta > 1:1502928/1‑68 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATaaa < 1:3699622/60‑1 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATaaa < 1:2715955/60‑1 (MQ=255) tCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATaaa < 1:3544779/60‑1 (MQ=255) tCGACGCATCTTCCTCATTCCTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGAt < 1:99817/69‑1 (MQ=255) cGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATaa > 1:1407492/1‑67 (MQ=255) gCATCTTCCTCATTCTTCTCTCCAAAAACCCCCTCATGCAATATAAACATCTATAAATAAAGATAAc > 1:39502/1‑67 (MQ=255) gCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATa > 1:2127181/1‑65 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAAtata > 1:1396980/1‑40 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAAtata > 1:619060/1‑40 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACa > 1:2793187/1‑63 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACa > 1:707419/1‑63 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACa > 1:3339319/1‑63 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACa > 1:2517429/1‑63 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:3576322/67‑1 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAg < 1:58046/67‑1 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:1258326/1‑69 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:915204/1‑69 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:1473066/1‑69 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:760962/1‑69 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:1871960/1‑69 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:605177/1‑69 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGaa > 1:2614193/1‑69 (MQ=255) ttCCTCATTCTTCTCTCCAAAAACCACCTCATGAAAtata > 1:148633/1‑40 (MQ=255) aTTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAatat > 1:2726037/1‑66 (MQ=255) aTTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTaa > 1:2680657/1‑69 (MQ=255) aTTCCTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTaa > 1:2194417/1‑69 (MQ=255) tcttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTaa > 1:3172055/1‑67 (MQ=255) cttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGcc > 1:563643/1‑69 (MQ=255) cttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGcc > 1:415676/1‑69 (MQ=255) cttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGcc > 1:2460678/1‑69 (MQ=255) cttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGcc > 1:2284175/1‑69 (MQ=255) cttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGcc > 1:1543108/1‑69 (MQ=255) cttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGcc > 1:710033/1‑69 (MQ=255) cttctCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGcc > 1:2942712/1‑69 (MQ=255) | TTCACATCACCTCCGCTATATGTAAAGCTAACGTTTCTGTGGCTCGACGCATCTTCCTCATTCTTCTCTCCAAAAACCACCTCATGCAATATAAACATCTATAAATAAAGATAACAATAGAATATTAAGCC > W3110S.gb/4547486‑4547616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |