| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,563,589 | T→A | 100% | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0% | 127.5 / NA | 43 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (18/25); total (18/25) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563524‑4563654 | tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2854277/69‑1 (MQ=255)tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:2652029/69‑1 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:101797/1‑69 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:3126402/1‑69 (MQ=255) ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:2210892/69‑1 (MQ=255) ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt > 1:2948428/1‑58 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTAGTGTCg > 1:1387983/1‑64 (MQ=255) gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt > 1:997492/1‑69 (MQ=255) tCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:1377255/51‑1 (MQ=255) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt < 1:2199710/63‑1 (MQ=255) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGATGTAACGCCATTTTACTGTCGCCCATGTgg < 1:2031203/67‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:2223213/69‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:1109192/69‑1 (MQ=255) acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg < 1:3188610/69‑1 (MQ=255) gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGAc < 1:1052322/69‑1 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt > 1:3512304/1‑36 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:598362/1‑37 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:1968352/1‑37 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa > 1:3379035/1‑37 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg > 1:2118114/1‑69 (MQ=255) tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:766091/69‑1 (MQ=255) tCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:677608/69‑1 (MQ=255) tCATAAATAGGCGGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg < 1:289039/69‑1 (MQ=255) cATAAATAGTCTGTAACGCCATTTTACTGTCGCCCa < 1:1620650/36‑1 (MQ=37) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:245708/36‑1 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:2179628/41‑1 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:2011859/41‑1 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:3182403/41‑1 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTgg < 1:112616/41‑1 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:3707962/1‑45 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:1025019/1‑45 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:2563563/1‑45 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:2372202/1‑44 (MQ=255) aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:2282746/1‑44 (MQ=255) aaaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:1853031/43‑1 (MQ=255) gCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCg > 1:3004709/1‑68 (MQ=255) cTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGcc < 1:2804896/69‑1 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:1804557/1‑46 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCa < 1:1647541/69‑1 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:410888/1‑46 (MQ=255) aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt > 1:1152743/1‑46 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCaa > 1:2257801/1‑66 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:1997806/68‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:1793327/68‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:760349/68‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg < 1:1027588/68‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg < 1:2091030/69‑1 (MQ=255) gCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg < 1:590012/69‑1 (MQ=255) | TCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563524‑4563654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |