| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,640,981 | C→T | 31.0% | A98V (GCT→GTT) | creB → | DNA‑binding response regulator in two‑component regulatory system with CreC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,640,981 | 0 | C | T | 31.0% | 37.2 / 21.0 | 29 | A98V (GCT→GTT) | creB | DNA‑binding response regulator in two‑component regulatory system with CreC |
| Reads supporting (aligned to +/- strand): ref base C (14/6); new base T (2/7); total (16/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.83e-01 | |||||||||||
TACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGC > W3110S.gb/4640915‑4641046 | tACCTGTACTGTTCCTGACGTCCCTAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTg < 1:2316448/69‑1 (MQ=37) aCCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCt > 1:3295007/1‑67 (MQ=255) gTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTgacgac > 1:3591256/1‑69 (MQ=255) tACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACt > 1:3029448/1‑69 (MQ=255) ccTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTgg > 1:2964336/1‑68 (MQ=255) gACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGCTa < 1:929493/68‑1 (MQ=255) gACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTaa < 1:1472542/69‑1 (MQ=255) aaGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGCTAAACCGTTTTc < 1:350369/69‑1 (MQ=255) gTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACt > 1:399331/1‑48 (MQ=255) gTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt > 1:3432153/1‑66 (MQ=255) gTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt > 1:1054346/1‑66 (MQ=255) gTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt > 1:882896/1‑66 (MQ=255) gTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt > 1:738610/1‑66 (MQ=255) gTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt > 1:3537710/1‑66 (MQ=255) aaGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGtttt > 1:179617/1‑63 (MQ=255) gTCGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGaa < 1:2958831/69‑1 (MQ=255) cGATCGCCTGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGCTAAAcc < 1:660893/51‑1 (MQ=255) cTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc < 1:2270928/69‑1 (MQ=255) cTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc < 1:446118/69‑1 (MQ=255) tGCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCa < 1:3542345/69‑1 (MQ=255) gCTTGGGCTGGAAATTGGTGTTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCa < 1:2992785/68‑1 (MQ=255) cTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAgg > 1:2185718/1‑69 (MQ=255) gggCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc > 1:3281123/1‑63 (MQ=255) gggCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc > 1:2103832/1‑63 (MQ=255) gggCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGcc > 1:2693791/1‑63 (MQ=255) gCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTgcg > 1:3719970/1‑69 (MQ=255) gCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTg < 1:1493770/67‑1 (MQ=255) tGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCa < 1:2428101/69‑1 (MQ=255) tGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGc < 1:2562566/68‑1 (MQ=255) | TACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGC > W3110S.gb/4640915‑4641046 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |