| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 408,996 | G→A | 39.1% | A83V (GCG→GTG) | rdgC ← | DNA‑binding protein, non‑specific |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 408,996 | 0 | G | A | 39.1% | 14.2 / 21.6 | 23 | A83V (GCG→GTG) | rdgC | DNA‑binding protein, non‑specific |
| Reads supporting (aligned to +/- strand): ref base G (7/7); new base A (3/6); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATT > W3110S.gb/408937‑409063 | cGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTa < 1:971865/68‑1 (MQ=255)cGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTa < 1:3505194/68‑1 (MQ=255) gTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGAtttttt < 1:823227/69‑1 (MQ=255) gTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGAtttttt < 1:556386/69‑1 (MQ=255) tCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTttct > 1:3661727/1‑69 (MQ=255) tCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTttct > 1:336213/1‑69 (MQ=255) ccGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGAtttt > 1:2558370/1‑64 (MQ=255) tCCAGTTTGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTg < 1:3349817/69‑1 (MQ=255) ttGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGAc > 1:2819078/1‑42 (MQ=255) ttGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACgg > 1:985404/1‑44 (MQ=255) ttGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACgg < 1:3025638/44‑1 (MQ=255) ttGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACgg > 1:2362117/1‑44 (MQ=255) ttGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACgg > 1:2267501/1‑44 (MQ=255) ttGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACgg < 1:2099100/44‑1 (MQ=255) ttGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACgg < 1:1590389/44‑1 (MQ=255) tGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcg > 1:2818222/1‑66 (MQ=255) tGGCGATTTTCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTgcgcg > 1:3450624/1‑66 (MQ=255) tttCGCTTCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAAc > 1:2042519/1‑69 (MQ=255) ttCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAAttt < 1:2612572/68‑1 (MQ=255) ttCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAAttt < 1:2467278/68‑1 (MQ=255) ttCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAAttt < 1:1690180/68‑1 (MQ=255) ttCCAGCACCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAAttt < 1:1555513/68‑1 (MQ=255) aGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGAcc < 1:3583458/68‑1 (MQ=255) gcCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCAtt > 1:1408238/1‑68 (MQ=255) | CGGTTTTCTTCAGCTTACGCGCCTGTTCCGCTTCCAGTTTGGCGATTTTCGCTTCCAGCGCCTGTTTAATCACCGGAGACGGGAGGATTTTTTCTTCTTTGCGCGCGCAGATAACAATTTGACCATT > W3110S.gb/408937‑409063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |