| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 419,576 | G→A | 20.8% | A254A (GCG→GCA) | brnQ → | predicted branched chain amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 419,576 | 0 | G | A | 20.8% | 46.9 / 8.4 | 24 | A254A (GCG→GCA) | brnQ | predicted branched chain amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base G (7/12); new base A (2/3); total (9/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
TGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGC > W3110S.gb/419515‑419642 | tGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTgg > 1:813004/1‑69 (MQ=255)tGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCATCGCTgg > 1:308157/1‑69 (MQ=255) ggTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTCGTCGAt < 1:480891/69‑1 (MQ=255) ttGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCa > 1:733948/1‑67 (MQ=255) ttGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGt > 1:2294610/1‑69 (MQ=255) ttGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGt > 1:3680183/1‑69 (MQ=255) ttGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTAAGACAGCGCGTCGCTGGTCGATCAg > 1:1591737/1‑68 (MQ=255) ggTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTc > 1:2261042/1‑68 (MQ=255) ggTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTc < 1:456673/68‑1 (MQ=255) gTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCgctgc < 1:2967887/69‑1 (MQ=255) gcTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGgcgc < 1:1569456/57‑1 (MQ=255) gcTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:1646078/67‑1 (MQ=255) gcTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:1893540/67‑1 (MQ=255) gcTGTTCCGACTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:1086448/67‑1 (MQ=255) cTGGGGTCAGACAGCGCGTCACTGGTCGATCAGTCTGCAAACGGCGCTGCTAt > 1:583045/1‑53 (MQ=255) cAGACAGCGCGTCGCTTGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAg > 1:3415002/1‑65 (MQ=255) aGACAGCGCGTCGCTGGTCTATCAGTCTGCAAACGGCGCTGCTATTCTg < 1:2977284/49‑1 (MQ=255) aCAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACTTTc < 1:2655582/60‑1 (MQ=255) cAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCtt < 1:328917/69‑1 (MQ=255) cAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCtt < 1:3150049/69‑1 (MQ=255) cAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCtt < 1:2415901/69‑1 (MQ=255) cAGCGCATCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCtt < 1:1371051/69‑1 (MQ=255) aGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACg < 1:260126/55‑1 (MQ=255) aGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACg < 1:1637782/55‑1 (MQ=255) gcgTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTggc > 1:2398171/1‑69 (MQ=255) | TGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGC > W3110S.gb/419515‑419642 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |