| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 470,819 | A→G | 55.7% | E320E (GAA→GAG) | mdlB → | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 470,819 | 0 | A | G | 55.7% | 20.6 / 79.1 | 61 | E320E (GAA→GAG) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base A (10/17); new base G (14/20); total (24/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01 | |||||||||||
AATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATC > W3110S.gb/470753‑470882 | aaTCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCt < 1:3653035/69‑1 (MQ=255) cGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGa > 1:2647551/1‑68 (MQ=255) aCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATgga < 1:218068/69‑1 (MQ=255) aCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATgga < 1:2065332/69‑1 (MQ=255) aCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATgga < 1:41796/69‑1 (MQ=255) aCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATgga > 1:1649741/1‑69 (MQ=255) aCTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATgga > 1:1940462/1‑69 (MQ=255) aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATggacgga > 1:2668061/1‑69 (MQ=255) aCCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacgga > 1:3024970/1‑69 (MQ=255) cACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATggacggac < 1:2990124/68‑1 (MQ=255) cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCa > 1:428255/1‑68 (MQ=255) cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCa > 1:869139/1‑68 (MQ=255) cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCa > 1:2730747/1‑68 (MQ=255) cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCa > 1:2215400/1‑68 (MQ=255) cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCa > 1:2311105/1‑68 (MQ=255) cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCa > 1:3393020/1‑68 (MQ=255) cAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCa > 1:2544456/1‑68 (MQ=255) ggCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGc > 1:2040372/1‑68 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:2623773/69‑1 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:3208975/69‑1 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:2753623/69‑1 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:75651/69‑1 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:3554995/69‑1 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:3618868/69‑1 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCaa < 1:485304/69‑1 (MQ=255) gCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCaa < 1:743182/69‑1 (MQ=255) gctgcAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCaa > 1:3392304/1‑64 (MQ=255) gttgCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAat < 1:1840046/57‑1 (MQ=255) gttgCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAat < 1:2724153/57‑1 (MQ=255) gttgCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGccc > 1:816937/1‑68 (MQ=255) gttgCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGAAATATGGCAATGATGATCGccc > 1:2769226/1‑68 (MQ=255) gCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGtt < 1:576258/68‑1 (MQ=255) ggTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACa < 1:3637581/68‑1 (MQ=255) ggTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACa < 1:1039681/68‑1 (MQ=255) ggTGAGCGCGTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACa < 1:2983138/68‑1 (MQ=255) ggTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:987048/69‑1 (MQ=255) ggTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:1420679/69‑1 (MQ=255) ggTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACa < 1:2162115/68‑1 (MQ=255) tGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAatgat < 1:1947079/52‑1 (MQ=255) cgcgTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatga > 1:304473/1‑47 (MQ=255) cgcgTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGc > 1:750722/1‑69 (MQ=255) cgcgTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGc > 1:1792139/1‑69 (MQ=255) gcgTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag > 1:2364058/1‑62 (MQ=255) gcgTGTTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag > 1:2631096/1‑62 (MQ=255) gtgtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:1595696/46‑1 (MQ=255) gtgtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:2737995/46‑1 (MQ=255) gtgtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:2028340/46‑1 (MQ=255) gtgtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:3685918/46‑1 (MQ=255) gtgtTTGAGCTGATGGACGGACCGCGCCAGCAATATGGCAatgatg < 1:399189/46‑1 (MQ=255) gtgtTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:3464490/60‑1 (MQ=255) gtgtTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACag < 1:1797007/60‑1 (MQ=255) gtgtTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCAcc < 1:526669/69‑1 (MQ=255) tgtTTGAGCTGATGGACGGACCTCGCCAGCAATATGGCAatgatt < 1:1019676/45‑2 (MQ=255) ggtTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCAcc < 1:3372124/67‑1 (MQ=255) tttGAACTGATGGACGGACCGCGCCAGCAATATGGCAatgatg > 1:1753776/1‑43 (MQ=255) tttGAACTGATGGACGGACCGCGCCAGCAATATGGCAatgatg > 1:3475686/1‑43 (MQ=255) tttGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATc < 1:2164020/69‑1 (MQ=255) tttGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATc < 1:3248610/69‑1 (MQ=255) tttGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATc < 1:1235843/69‑1 (MQ=255) tttGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATc < 1:2840331/69‑1 (MQ=255) tGAGCTGATGGACGGACAGCGCCAGCAATATGGCAatgat > 1:3514152/1‑40 (MQ=37) | AATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGCTGGTGAGCGCGTGTTTGAACTGATGGACGGACCGCGCCAGCAATATGGCAATGATGATCGCCCGTTACAGAGTGGCACCATC > W3110S.gb/470753‑470882 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |