Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 487,168 C→T 23.8% P470L (CCA→CTA)  kefA → fused mechanosensitive channel proteins

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb487,1680CT23.8% 37.1 / 7.4 21P470L (CCA→CTA) kefAfused mechanosensitive channel proteins
Reads supporting (aligned to +/- strand):  ref base C (7/9);  new base T (3/2);  total (10/11)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCA  >  W3110S.gb/487102‑487236
                                                                  |                                                                    
tGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTcc                                                                      >  1:1529658/1‑67 (MQ=255)
tGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTcc                                                                      >  1:2537247/1‑67 (MQ=255)
tGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCa                                                                     <  1:2290143/68‑1 (MQ=255)
        tCGAAAAACCTGAAATCCTTCCTGACTCAGCAAATCTTTTGGTTGAACAGTAACCGTcc                                                                      <  1:2702157/59‑1 (MQ=255)
        tCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTcc                                                                      <  1:1873448/59‑1 (MQ=255)
          gAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATggactggga                                                          >  1:2726838/1‑69 (MQ=255)
          gAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATgg                                                                 <  1:3323450/62‑1 (MQ=255)
          gAAAAACCTGAAATCCATCCTGACTCAGCAAATATTTTGGGTGAACAGTAACCGTCCAATggactggga                                                          >  1:2610561/1‑69 (MQ=255)
            aaaaCCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATggact                                                              >  1:1393002/1‑63 (MQ=255)
             aaaCCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATggactgggactg                                                       >  1:190408/1‑69 (MQ=255)
                       tccatccTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCg                                             >  1:3112951/1‑69 (MQ=255)
                             cTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCTAATGGACTGGGACTGGATCAAAGCGTTccc                                        >  1:2035791/1‑68 (MQ=255)
                                      cAAATCTTTTGGGTGAACAGTAACCGTCCAATggactg                                                             >  1:33325/1‑38 (MQ=255)
                                       aaaTCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGa                             <  1:972723/69‑1 (MQ=255)
                                          tCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGaaa                           <  1:542335/68‑1 (MQ=255)
                                              ttGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGc                                              >  1:145432/1‑45 (MQ=255)
                                                    gAACAGTAACCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTaa                >  1:3687156/1‑69 (MQ=255)
                                                    gAACAGTAACCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTa                 >  1:1242648/1‑68 (MQ=255)
                                                            aCCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGaa                            <  1:3692798/49‑1 (MQ=255)
                                                            aCCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGa        <  1:2418308/69‑1 (MQ=255)
                                                            aCCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGaaa                           <  1:618049/50‑1 (MQ=255)
                                                            aCCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGa        <  1:807311/69‑1 (MQ=255)
                                                            aCCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGa        <  1:2206665/69‑1 (MQ=255)
                                                              cGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCa                                     <  1:848923/38‑1 (MQ=255)
                                                              cGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGaaa      <  1:192540/69‑1 (MQ=255)
                                                                  cAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCa  >  1:3455866/1‑69 (MQ=255)
                                                                  cAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCa  >  1:598575/1‑69 (MQ=255)
                                                                  |                                                                    
TGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCA  >  W3110S.gb/487102‑487236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: