| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 487,168 | C→T | 23.8% | P470L (CCA→CTA) | kefA → | fused mechanosensitive channel proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 487,168 | 0 | C | T | 23.8% | 37.1 / 7.4 | 21 | P470L (CCA→CTA) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base C (7/9); new base T (3/2); total (10/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCA > W3110S.gb/487102‑487236 | tGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTcc > 1:1529658/1‑67 (MQ=255)tGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTcc > 1:2537247/1‑67 (MQ=255)tGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCa < 1:2290143/68‑1 (MQ=255) tCGAAAAACCTGAAATCCTTCCTGACTCAGCAAATCTTTTGGTTGAACAGTAACCGTcc < 1:2702157/59‑1 (MQ=255) tCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTcc < 1:1873448/59‑1 (MQ=255) gAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATggactggga > 1:2726838/1‑69 (MQ=255) gAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATgg < 1:3323450/62‑1 (MQ=255) gAAAAACCTGAAATCCATCCTGACTCAGCAAATATTTTGGGTGAACAGTAACCGTCCAATggactggga > 1:2610561/1‑69 (MQ=255) aaaaCCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATggact > 1:1393002/1‑63 (MQ=255) aaaCCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATggactgggactg > 1:190408/1‑69 (MQ=255) tccatccTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCg > 1:3112951/1‑69 (MQ=255) cTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCTAATGGACTGGGACTGGATCAAAGCGTTccc > 1:2035791/1‑68 (MQ=255) cAAATCTTTTGGGTGAACAGTAACCGTCCAATggactg > 1:33325/1‑38 (MQ=255) aaaTCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGa < 1:972723/69‑1 (MQ=255) tCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGaaa < 1:542335/68‑1 (MQ=255) ttGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGc > 1:145432/1‑45 (MQ=255) gAACAGTAACCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTaa > 1:3687156/1‑69 (MQ=255) gAACAGTAACCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTa > 1:1242648/1‑68 (MQ=255) aCCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGaa < 1:3692798/49‑1 (MQ=255) aCCGTCTAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGa < 1:2418308/69‑1 (MQ=255) aCCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGaaa < 1:618049/50‑1 (MQ=255) aCCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGa < 1:807311/69‑1 (MQ=255) aCCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGa < 1:2206665/69‑1 (MQ=255) cGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCa < 1:848923/38‑1 (MQ=255) cGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGaaa < 1:192540/69‑1 (MQ=255) cAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCa > 1:3455866/1‑69 (MQ=255) cAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCa > 1:598575/1‑69 (MQ=255) | TGAGTGTGTCGAAAAACCTGAAATCCATCCTGACTCAGCAAATCTTTTGGGTGAACAGTAACCGTCCAATGGACTGGGACTGGATCAAAGCGTTCCCGCAAAGCCTGAAAGATGAATTTAAGTCGATGAAAATCA > W3110S.gb/487102‑487236 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |