| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 488,526 | C→T | 23.2% | L923F (CTT→TTT) | kefA → | fused mechanosensitive channel proteins |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 488,526 | 0 | C | T | 23.2% | 137.7 / 38.9 | 69 | L923F (CTT→TTT) | kefA | fused mechanosensitive channel proteins |
| Reads supporting (aligned to +/- strand): ref base C (24/29); new base T (12/4); total (36/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.79e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01 | |||||||||||
GTGTTCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTATTCGAACGTCCG > W3110S.gb/488460‑488594 | gtgtTCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCtt < 1:1410315/69‑1 (MQ=255) ttCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCttggt > 1:865522/1‑69 (MQ=255) tCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCttggttttgg > 1:3279887/1‑68 (MQ=255) cGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGa < 1:1930894/69‑1 (MQ=255) cGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGa < 1:2972838/69‑1 (MQ=255) cGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGa > 1:1987701/1‑69 (MQ=255) gggATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTc > 1:2571023/1‑68 (MQ=255) gggATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTc > 1:1421931/1‑68 (MQ=255) gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:1428916/1‑69 (MQ=255) gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:3327213/1‑69 (MQ=255) gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:1220671/1‑69 (MQ=255) gATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:2362886/1‑69 (MQ=255) aaaCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt < 1:169969/66‑1 (MQ=255) aaaCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt < 1:1962653/66‑1 (MQ=255) aaCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACt > 1:3358038/1‑69 (MQ=255) aCTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGaaa < 1:2826014/56‑1 (MQ=255) cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:1003560/57‑1 (MQ=255) cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:2573322/57‑1 (MQ=255) cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:79218/57‑1 (MQ=255) cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAAtt < 1:1242613/57‑1 (MQ=255) cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCTGTAACTTc < 1:2820154/69‑1 (MQ=255) cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt < 1:2535051/63‑1 (MQ=255) cTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTc < 1:981728/69‑1 (MQ=255) cAGTGGCTGGCCGCAGCATTATCCGTAGGTCttggt > 1:3292166/1‑36 (MQ=255) cAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGtc > 1:1829842/1‑69 (MQ=255) tggctggcCGCAGCATTATCCGTAGGTCttggttttggt < 1:2862261/39‑1 (MQ=255) tggctggcCGCAGCATTATCCGTAGGTCttggttttggt < 1:1090845/39‑1 (MQ=255) tggctggcCGCAGCATTATCCGTAGGTCttggttttggt < 1:2232771/39‑1 (MQ=255) ggctggcCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCg > 1:1925575/1‑69 (MQ=255) cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTg < 1:1323852/67‑1 (MQ=255) cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTg < 1:1956525/67‑1 (MQ=255) cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:442615/69‑1 (MQ=255) cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:848707/69‑1 (MQ=255) cGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:927947/69‑1 (MQ=255) gcagcaTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGAt < 1:2899112/68‑1 (MQ=255) caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:228123/69‑1 (MQ=255) caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:1917903/69‑1 (MQ=255) caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:1249509/69‑1 (MQ=255) caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:2806199/69‑1 (MQ=255) caTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:2024464/69‑1 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:2988575‑M1/3‑26 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg < 1:3242849‑M1/40‑17 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:2450555‑M1/3‑26 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:797325‑M1/3‑26 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:3060973‑M1/3‑26 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:379962‑M1/3‑26 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:1171766‑M1/3‑26 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:870414‑M1/3‑26 (MQ=255) gatatCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:897869‑M1/3‑26 (MQ=255) aTTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc < 1:95966/68‑1 (MQ=255) ttATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCt > 1:1848944/1‑68 (MQ=255) ttATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTa > 1:2641476/1‑69 (MQ=255) tATCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt > 1:927043‑M1/1‑24 (MQ=255) tATCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt > 1:1678224‑M1/1‑24 (MQ=255) tATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTa > 1:2015671/1‑68 (MQ=255) aTCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt < 1:3281144‑M1/40‑18 (MQ=255) aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGt > 1:840139/1‑40 (MQ=255) aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTa > 1:2258341/1‑67 (MQ=255) aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2328173/1‑68 (MQ=255) aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:3046144/1‑68 (MQ=255) aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2853629/1‑68 (MQ=255) aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2597303/1‑68 (MQ=255) aTCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTAt > 1:2176695/1‑68 (MQ=255) tCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:430822‑M1/1‑22 (MQ=255) tCCGTTGGTTTTGGTTTCGGTTgtgaaaagttttttgg > 1:443368‑M1/1‑22 (MQ=255) tCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTc > 1:1964438/1‑64 (MQ=255) ccGTTGGTTTTGGTTTCGGTTgtgaaaagtttttt < 1:3031426‑M1/35‑15 (MQ=255) cGTTGGTTTTGGTTTCGGTTgtgaaaagttttttggt < 1:2979144‑M1/37‑18 (MQ=255) tCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTATTCGAACGTc < 1:2536898/68‑1 (MQ=255) cTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTc > 1:3220261/1‑36 (MQ=255) cTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCgg < 1:3079877/44‑1 (MQ=255) cTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTATTCGAACGTCCg > 1:3463949/1‑69 (MQ=255) cTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTATTCGAACGTCCg > 1:1507504/1‑69 (MQ=255) cTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTATTCGAACGTCCg > 1:2654397/1‑69 (MQ=255) | GTGTTCGGATCGCTGGGCGTCTCTTGGGATAAACTCCAGTGGCTGGCCGCAGCATTATCCGTAGGTCTTGGTTTTGGTTTACAAGAAATTTTCGGTAACTTCGTCTCCGGTTTGATCATTCTATTCGAACGTCCG > W3110S.gb/488460‑488594 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |