| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 495,162 | G→A | 21.7% | E273E (GAG→GAA) | htpG → | molecular chaperone HSP90 family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 495,162 | 0 | G | A | 21.7% | 44.6 / 8.2 | 23 | E273E (GAG→GAA) | htpG | molecular chaperone HSP90 family |
| Reads supporting (aligned to +/- strand): ref base G (13/5); new base A (2/3); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAA > W3110S.gb/495095‑495225 | aaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAg < 1:122663/68‑1 (MQ=255)aaCACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAg < 1:1443077/68‑1 (MQ=255) tCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCa > 1:1459794/1‑69 (MQ=255) tCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCa > 1:1553768/1‑69 (MQ=255) tCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCa > 1:305384/1‑69 (MQ=255) tCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCa > 1:2645049/1‑69 (MQ=255) cACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACGAGCctg < 1:3222014/69‑1 (MQ=255) cGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTccc < 1:2844497/69‑1 (MQ=255) tGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCg > 1:286034/1‑69 (MQ=255) ggAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCctgctg < 1:2181003/47‑1 (MQ=255) ggAGCCACAACCGTGTTGAAGGTAAGCAGGAATACACCAGCctgctg < 1:2435260/47‑1 (MQ=255) acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGata > 1:3226673/1‑69 (MQ=255) acaACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGata > 1:2353383/1‑69 (MQ=255) caACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGata < 1:2193064/68‑1 (MQ=255) gtgtTGAAGGTAAGCAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGa < 1:3130041/69‑1 (MQ=255) gtTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGaa > 1:1357830/1‑68 (MQ=255) tGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAAcc > 1:3698549/1‑68 (MQ=255) tGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAAcc > 1:2799847/1‑68 (MQ=255) tGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAAcc > 1:2201533/1‑68 (MQ=255) gAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACcgc > 1:3692928/1‑69 (MQ=255) aGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa < 1:2636133/69‑1 (MQ=255) gCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa < 1:1127624/68‑1 (MQ=255) cAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATaaa > 1:2391031/1‑69 (MQ=255) cAGGAATACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATaaa > 1:883667/1‑69 (MQ=255) gAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATa > 1:3600788/1‑64 (MQ=255) | AACACATCGCCCACGACTTTAATGATCCGCTGACCTGGAGCCACAACCGTGTTGAAGGTAAGCAGGAGTACACCAGCCTGCTGTACATCCCGTCCCAGGCTCCGTGGGATATGTGGAACCGCGATCATAAA > W3110S.gb/495095‑495225 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |